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Evaluation of the excretion of Zika Virus in serum, urine and saliva of pediatric patients using Real Time PCR method

Grant number: 16/11022-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2016
Effective date (End): July 31, 2017
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Edison Luiz Durigon
Grantee:Vanessa Barbosa da Silveira
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The Zika virus (ZIKV) is an arbovirus belonging to the Flaviviridae family, transmission can be made by several species of Aedes mosquitoes. The clinical symptoms of ZIKV infection are similar to Dengue and Chikungunya, with mainly headaches, fever, myalgia, arthralgia, conjutival hyperemia and maculopapular rash. The knowledge about pathology, combination with other agents, virus biology and disease are still unknown. However there is no consensus on the best biological material for the detection of ZIKV in acute patients. The aims of our project is to compare three body fluids (Serum, Swab Oral and Urine) of pediatric patients with symptoms of unknown rash illness, with less than 7 days of the onset of disease (acute phase) to determine which material has the largest amount virus circulating virus at each period of the disease. The comparison will be performed by RT-PCR with primers and probe previously described by Lanciotti et al., 2007.