Subcellular partitioning and specific biomarkers to trace metals exposure in the mangrove sentinel Ucides cordatus (Linnaeus, 1763).

• Grant number: 16/05414-6
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: December 01, 2016
• End date: November 30, 2017
• Field of knowledge: Biological Sciences - Zoology - Morphology of Recent Groups
• Principal Investigator: Camilo Dias Seabra Pereira
• Grantee: Luis Felipe de Almeida Duarte
• Supervisor: Julian Blasco Moreno
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Institution abroad: Consejo Superior de Investigaciones Científicas (CSIC), Spain
• Associated to the scholarship: 14/13448-2 - Biological tolerance, sublethal effects and bioaccumulation in Ucides cordatus (Linnaeus, 1763) exposed to contaminants, via food and water, BP.PD

Abstract

This research project to Bolsa Estágio de Pesquisa no Exterior (BEPE) aims to complement the main Post Doctorate project in progress. It would be conducted at Institute of Marine Sciences of Andalusia (ICMAN-CSIC) under the supervision of Dr. Julián Blasco. Briefly, this proposal is focused on adapt and employ new methodologies regarding subcellular metal partitioning (SMP) and specific biomarkers of defense and effects (Metallothioneins [MTs] and Aminolevulinic Acid Dehydratase [ALA-D], respectively) in Ucides cordatus tissues (hepatopancreas and anterior-posterior gills) during long-term exposure to non-essential metals (cadmium and lead) via water and food. Understanding process of metal detoxification and effects through proteins (MTs), enzymatic activity (ALA-D) and storage in metabolically inactive forms and their metabolically active concentration within an organism (SMP) may considerably improve the ability to predict the toxicity of metals and, obviously, different biological tolerances may be demonstrated. Therefore, the main objective of the study at ICMAN-CSIC is to adapt and to employ new protocols for quantifying SMP, MTs and ALA-D in Ucides cordatus tissues after three different exposure experiments (Exp.) conducted at UNIFESP: non-essential metals via water (Cd [Exp.1] and Pb [Exp.2]) and food contaminated (Rhizophora mangle leaves [Exp. 3]; the main source of food to this crustacean). It is expected that these data will help to clarify detoxification process, sublethal damages and biological tolerances addressed in the main project. The new adapted protocols will improve the reliability and consistency of analysis in this sentinel species, which could be employed in future environmental and ecological studies. In addition, our study will provide reliable information for the proper management of mangrove ecosystems.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ALMEIDA DUARTE, LUIS FELIPE; BLASCO MORENO, JULIAN; MIRANDA CATHARINO, MARILIA GABRIELA; GONCALVES MOREIRA, EDSON; TROMBINI, CHIARA; SEABRA PEREIRA, CAMILO DIAS. Mangrove metal pollution induces biological tolerance to Cd on a crab sentinel species subpopulation. Science of The Total Environment, v. 687, p. 768-779, OCT 15 2019. (14/13448-2, 10/01552-9, 16/05414-6)

FERNANDA AUGUSTO MOSCHETTO; ROBERTO PEREIRA BORGES; LUIS FELIPE DE ALMEIDA DUARTE. Population structure of Callichirus major (Say 1818) (Crustacea: Callianassidae) and conservation considerations at Southeast coast of São Paulo, Brazil. Anais da Academia Brasileira de Ciências, v. 92, n. 1, 2020-02-17. (14/13448-2, 16/05414-6)

DE ALMEIDA DUARTE, LUIS FELIPE; BLASCO, JULIAN; MIRANDA CATHARINO, MARILIA GABRIELA; MOREIRA, EDSON GONCALVES; TROMBINI, CHIARA; NOBRE, CAIO RODRIGUES; MORENO, BEATRIZ BARBOSA; DE SOUZA ABESSA, DENIS MOLEDO; SEABRA PEREIRA, CAMILO DIAS. Lead toxicity on a sentinel species subpopulation inhabiting mangroves with different status conservation. Chemosphere, v. 251, JUL 2020. (14/13448-2, 16/05414-6, 10/01552-9)