Evaluation of direct and indirect ischemic pre-conditioning effects in young rat livers

• Grant number: 16/10689-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2016
• Effective date (End): August 31, 2017
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Ana Cristina Aoun Tannuri
• Grantee: Pedro Filipe Medeiros Gomes
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Liver transplantation (LT) today stands as the only effective and definitive treatment for end-stage liver failure. However, the ischemia-reperfusion (I/R) lesion, an always present kind of injury, is a major cause of primary dysfunction and is associated with the occurrence of acute and chronic rejection. In order to increase TH's success, methods have been developed to diminish I/R damage. Among them, the ischemic preconditioning technique (IPC) stands out as a promising method to decrease I/R injury. This project aims to evaluate the local and multisystemic effects of the I/R lesion associated with two IPC techniques: direct IPC and remote IPC, as well as to compare the two techniques with each other and with the absence of IPC execution in young (4-5 weeks) Wistar rat models. Therefore, liver, heart, lung, kidney and small intestine biopsies will be harvested after the surgical proceedings to histological and biomolecular analysis of these organs: the first one in order to assess the magnitude of the structural damage and the second to assess the expression of apoptosis-related genes: Bax (pro-apoptotic) and Bcl-XL (anti-apoptotic), cell proliferation related gene: (IL-6) gene, and the eNOS (endothelial nitricoxide synthase). Also, blood samples will be harvested to quantify SGOT, SGPT, gamma-GT, alkaline phosphatase and total and fractionated bilirubin.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES, P. F. M.; TANNURI, A. C. A.; NOGUEIRA, T. M.; LUAMOTO, L. R.; PAES, V. R.; COELHO, M. C. M.; GONCALVES, J. O.; SERAFINI, S.; TANNURI, U.. Remote Ischemic Preconditioning Is Efficient in Reducing Hepatic Ischemia-Reperfusion Injury in a Growing Rat Model and Does Not Promote Histologic Lesions in Distant Organs. Transplantation Proceedings, v. 50, n. 10, p. 3840-3844, DEC 2018. (16/10689-4)