The application of low power laser to accelerate the ossification> histological and immunohistochemical analysis

• Grant number: 16/07023-4
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2016
• Effective date (End): December 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal researcher: Maria Bernadete Sasso Stuani
• Grantee: Natália Claudino de Oliveira
• Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Low-level laser therapy (LBP) has been used Dentistry with various goals, especially to reduce the time surgical wound healing. Objective: The aim of this in vivo study will evaluate qualitatively and quantitatively the effect of irradiation in LBPprocess of bone remodeling after tooth extraction in young rats. Material and Methods: 30 Wistar rats will be randomly distributed in following groups: control group (n = 15) animals with tooth extraction without application of LBP, and experimental group (n = 15) animals that have extraction Dental (Ex) and application of LBP (Ex + LBP) in the first three days (54 J / cm2 forday). The animals will be sacrificed at 5, 7 and 10 days after dental extraction procedure. In this study, the effects will be analyzed laser application on wound healing using light microscopy, light polarized and immunostaining. For this will be assessed the following parameters: 1)percentage of bone formation within the socket; 2) degree of process inflammatory; 3) the degree of maturation of collagen; 4) immunostaining TRAP, RANK-L and OPG. All results will be subjected to analysis statistically using the ANOVA test and the Mann-Whitney U test (p <0.05).