Mesenchymal stem cells derived from Wharton's Jelly in cardiopulmonary injury and systemic neuroimmunomodulation in sepsis

• Grant number: 15/21308-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2016
• End date: May 31, 2018
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Samirah Abreu Gomes
• Grantee: José Manuel Cóndor Capcha
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 10/19012-0 - Evaluation of hematopoietic stem cell treatment in dogs with chronic renal failure, AP.TEM

Abstract

Sepsis is a disease related to the presence of infection with a systemic inflammatory response, its pathophysiology involves a complex network of cytokines and inflammatory mediators that cause injury to various tissues. Currently there are many attempts to reduce mortality, although there is not specific strategy for treating the disease. In recent years, stem cells have received great interest in the treatment of inflammatory and degenerative diseases; in the last decade many studies have shown that mesenchymal stem cells (MSCs) have immunosuppressive and immunomodulatory properties, and that they can protect the inflammatory effect during endotoxemia. It is currently already being used MSC as a last resource in a number of critical illnesses, although there are still some controversies in the results. About the treatment of sepsis with stem cells, first, is necessary the expansion of pre-clinical studies before of its application in clinical practice. With the knowledge of the properties of CTM and its viability in interspecies therapy, our aim is to study the effect of mesenchymal stem cells from human Wharton jelly in an experimental sepsis model "rats" (ligation and puncture Ceco, LPC). The animals will be randomized into three groups: LPC (group of sepsis induction), LPC + CTM (group with induction of more sepsis treatment with stem cells) and SHAM (control group). The groups will submitted to cardiac, pulmonary, immune and autonomic evaluation, beyond the mechanisms involved as result of treatment; finally, the data obtained will be evaluate to analysis of variance, followed by Newman-Keuls test. The degree of significance for statistical analysis will be p<0.05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONDOR CAPCHA, JOSE MANUEL; RODRIGUES, CAMILA ELEUTERIO; MOREIRA, ROBERTO DE SOUZA; SILVEIRA, MARCELO DUARTE; DOURADO, PAULO; DOS SANTOS, FERNANDO; IRIGOYEN, MARIA CLAUDIA; JENSEN, LEONARDO; GARNICA, MARGOTH RAMOS; NORONHA, IRENE L.; et al. Wharton's jelly-derived mesenchymal stem cells attenuate sepsis-induced organ injury partially via cholinergic anti-inflammatory pathway activation. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, v. 318, n. 1, p. R135-R147, JAN 2020. (10/19012-0, 15/21308-9)

CONDOR CAPCHA, JOSE MANUEL; MOREIRA, ROBERTO S.; RODRIGUES, CAMILA E.; SILVEIRA, MARCELO A. D.; ANDRADE, LUCIA; GOMES, SAMIRAH A.. Using the Cecal Ligation and Puncture Model of Sepsis to Induce Rats to Multiple Organ Dysfunction. BIO-PROTOCOL, v. 11, n. 7, APR 5 2021. (15/21308-9, 10/19012-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAPCHA, José Manuel Cóndor. Wharton\'s Jelly derived mesenchymal stem cells in sepsis-induced cardiopulmonar injury and systemic neuroimmunomodulation. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.