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Color analysis of dental enamel submitted to Nd:YAG laser applied for erosion prevention

Grant number: 16/04440-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2016
Effective date (End): August 31, 2017
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Maria Angela Pita Sobral Fernandes
Grantee:Juliana Pereira Rodrigues
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The literature proposes the Nd: YAG laser as alternative method to protect the dental enamel of erosive challenges that occur in the oral cavity. This laser promotes melting of the crystal structure of enamel making it more resistant to the action of acids. This effect may change the color when irradiated enamel. The objective of this study is verify if the enamel changes color when subjected to Nd: YAG laser. Forty crowns of human incisors are sectioned longitudinally and transversely to obtain specimens of the buccal surface. They will be randomly divided into four groups as follows (n = 10): control group (no treatment) (C ) , group acidulated phosphate fluoride (APF) (pH 3.5, 1.23% F, por 4min), Nd laser group: YAG (100 mJ , 1 W, 10 Hz, 141.5 J / cm2) , and APF + Nd: YAG laser. The laser beam is applied by contact perpendicularly sweeping the surface for 30 seconds. The color will be measured before and after treatment and in a ultraviolet visible light spectrophotometer (Konica Minolta CM3700A) according to CIELAB parameters of the system (Commission Internationale de I'clairage L *, a *, b *). Standard measurement for reflection, light source with a wavelength of 400-700 nm, D65 illuminant, observer 2o. and black colored background will be used. Each specimen is measured three times by the same researcher and a final average obtained. Statistical analysis of the data obtained will be used the ANOVA and Tukey test for comparison between groups (p <0.05).

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