Transcriptomics in placentas of women carrier of sickle cell disease.

• Grant number: 15/08330-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2016
• End date: March 31, 2019
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Mônica Barbosa de Melo
• Grantee: Letícia de Carvalho Baptista
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 14/00984-3 - Red blood cell disorders: pathophysiology and new therapeutic approaches, AP.TEM

Abstract

The sickle cell disease (SCD) are a group of genetic disorders that have in common that the presence of hemoglobin (Hb) S, a mutant protein that undergoes polymerization under low oxygen tensions, generating vaso-occlusion. The obstruction and the lesion of the vascular endothelium and tissue cause an inflammatory response that in turn results in a chronic inflammatory state in patients with PD. Pregnancy in this disease is accompanied by an increased incidence of episodes of pain, infection, pyelonephritis, pulmonary complications, thromboembolic events and preeclampsia. Maternal placental circulation is susceptible to vaso-occlusion, which can contribute to the areas of fibrosis, necrosis of villi and infarction in the placenta.However, the mechanisms responsible for the onset of these complications and the physiopathology involved are still largely unexplored. Hypothesized there may be disruption in the expression of some roads as inflammatory, endothelial and oxidative stress due to polymerization of Hb S. Therefore, we propose a prospective, case-control, with evaluation of five groups: Group 1 pregnant women with complications and SCD (Hb SS, n = 3), group 2 pregnant women with uncomplicated SCD (Hb SS, n = 3), group 3 pregnant women with complications and SCD (Hb SC, n = 3), Group 4 pregnant women with uncomplicated SCD (Hb SC, n = 3) and Group 5 pregnant women without SCD (Hb AA n = 3). The central objective of the research is to analyze the gene expression on a large scale by RNA-Seq technique in placentas of women with DF. This technique can provide important data for a better understanding of how the disease affects the physiology of the placenta and increases the risk of maternal and fetal complications.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BAPTISTA, LETICIA C.; FIGUEIRA, CAMILLA O.; SOUZA, BRUNO B.; FERTRIN, KLEBER Y.; ANTOLINI, ARTHUR; COSTA, FERNANDO F.; DE MELO, MONICA B.; COSTA, MARIA LAURA. Highlight Article: Different morphological and gene expression profile in placentas of the same sickle cell anemia patient in pregnancies of opposite outcomes. Experimental Biology and Medicine, v. 244, n. 5, p. 395-403, APR 2019. (15/08330-5, 14/00984-3)

BAPTISTA, LETICIA CARVALHO; COSTA, MARIA LAURA; SURITA, FERNANDA GARANHANI; ROCHA, CRISTIANE DE SOUZA; LOPES-CENDES, ISCIA; DE SOUZA, BRUNO BATISTA; COSTA, FERNANDO FERREIRA; DE MELO, MONICA BARBOSA. Placental transcriptome profile of women with sickle cell disease reveals differentially expressed genes involved in migration, trophoblast differentiation and inflammation. BLOOD CELLS MOLECULES AND DISEASES, v. 84, SEP 2020. (15/08330-5, 14/00984-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BAPTISTA, Letícia de Carvalho. Transcriptomics in placentas of women with sickle cell disease. 2019. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.