Clinical comparison of the effectiveness the rotary systems hyflex CM and protaper next for the removal of endotoxins and cultivable bacteria from primary infected root canals

• Grant number: 16/08157-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2016
• End date: June 30, 2018
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Rogerio de Castilho Jacinto
• Grantee: Camila Ambrósio Dias
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Introduction: This clinical study will be conducted to compare the effectiveness of two rotary systems Hyflex CM (Coltene-Whaledent, Allstetten, Switzerland) and Protaper Next (Dentsply Maillefer, Ballaigues, Switzerland) in removing endotoxins and cultivable bacteria from primarily infected root canals. Methods: Twenty-four primarily infected root canals of maxillary molars will be selected patients seeking for endodontic treatment and randomly divided into 2 groups: Hyflex CM (Coltene-Whaledent,) (n = 12); and protaper Next (Dentsply Maillefer) (n = 12). Samples will be collected will sterile apyrogenic paper points before and after chemomechanical preparation of the mesio-buccal canal and inoculated in specific vials according to the analysis to be performed - endotoxin or microbiological test. The irrigation will be performed by using 2.5% sodium hypochlorite. A chromogenic limulus amebocyte lysate assay test will be used to quantify endotoxins. Culture techniques will used to determine bacterial colony-forming unit counts. Colected data from CFU and endotoxin concentration will be statistically analysed using SPSS for Windows (SPSS Inc, Chicago, IL). Significance level will be 5% (P <0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIAS MACHADO, CAMILA AMBROSIO; ANDOLFATTO SOUZA, AMANDA CASELATO; LOUREIRO, CAROLINE; MARTINHO, FREDERICO CANATO; ANGELO CINTRA, LUCIANO TAVARES; DEZAN JUNIOR, ELOI; JACINTO, ROGERIO DE CASTILHO. Comparison of two rotary systems in bacteria/lps removal from endodontic infections: randomized clinical trial. Brazilian Oral Research, v. 33, 2019. (16/08157-4)

DIAS MACHADO, CAMILA AMBROSIO; ANDOLFATTO SOUZA, AMANDA CASELATO; LOUREIRO, CAROLINE; MARTINHO, FREDERICO CANATO; ANGELO CINTRA, LUCIANO TAVARES; DEZAN JUNIOR, ELOI; JACINTO, ROGERIO DE CASTILHO. Comparison of two rotary systems in bacteria/lps removal from endodontic infections: randomized clinical trial. BRAZILIAN ORAL RESEARCH, v. 33, p. 9-pg., 2019-01-01. (16/08157-4)