Role of microRNA in skin wound healing: effects of low level laser therapy and comparative study with oral mucosa healing

• Grant number: 16/16332-0
• Support Opportunities: Scholarships abroad - Research
• Start date: February 01, 2017
• End date: January 31, 2018
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Alyne Simões Gonçalves
• Grantee: Alyne Simões Gonçalves
• Host Investigator: Luisa Dipietro
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of Illinois at Chicago (UIC), United States

Abstract

Wound healing is an intricately orchestrated process. The attainment of perfect healing remains one of the holy grails of the wound repair field, and impaired and aberrant wound healing is a significant clinical problem, affecting approximately 6.5 milion people in the United States. One tissue that nearly always exhibits superior wound healing is the oral mucosa. It has been suggested that the site-specific difference of wound healing of skin and oral mucosa is due to the environmental variation of two sites. However, skin transposed into oral cavity maintains its morphologic characteristics and may result in intraoral keloids, suggesting that rapid wound healing in oral mucosa is likely to involve intrinsic characteristics of mucosal tissue rather than arising simply from environmental factors. MicroRNAs, non-protein-coding small endogenous RNAs have been shown to regulate many developmental, physiological and disease processes, including wound healing. In addition, they may also be involved with the biomodulatory effect of Low Level Laser Therapy (LLLT). In this proposal, we will explore the microRNAs associated with enhanced wound closure in oral mucosa, and will test the feasibility of accelerating skin wound closure by applying mucosa specific microRNA to the skin wound. In addition, we will study the microRNA expression in skin wound of rats, which will be or will not be treated with LLLT. This study will provide a foundation for developing novel microRNA-based therapeutic approaches to promote wound closure and/or prevent chronic wounds, as well as will provide important information regarding the action mechanism of LLLT related to its biomodulatory effect. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SIMOES, ALYNE; CHEN, LIN; CHEN, ZUJIAN; ZHAO, YAN; GAO, SHANG; MARUCHA, PHILLIP T.; DAI, YANG; DIPIETRO, LUISA A.; ZHOU, XIAOFENG. Differential microRNA profile underlies the divergent healing responses in skin and oral mucosal wounds. SCIENTIFIC REPORTS, v. 9, MAY 9 2019. (16/16332-0)

CHEN, LIN; SIMOES, ALYNE; CHEN, ZUJIAN; ZHAO, YAN; WU, XINMING; DAI, YANG; DIPIETRO, LUISA A.; ZHOU, XIAOFENG. Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 15, AUG 2019. (16/16332-0)

DE CASTRO, JULIANA RODRIGUES; PEREIRA, FILIPI DA SILVA; CHEN, LIN; ARANA-CHAVEZ, VICTOR ELIAS; BALLESTER, RAFAEL YAGUE; DIPIETRO, LUISA A.; SIMOES, ALYNE. Improvement of full-thickness rat skin wounds by photobiomodulation therapy (PBMT): A dosimetric study. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 206, MAY 2020. (16/16332-0, 14/21214-1)

CHEN, LIN; CHEN, ZUJIAN; SIMOES, ALYNE; WU, XINMING; DAI, YANG; DIPIETRO, LUISA A.; ZHOU, XIAOFENG. Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 2, JAN 2 2020. (16/16332-0)