Characterization of dermis extracellular matrix of Wistar rats

• Grant number: 16/20008-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2016
• End date: October 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Maria Angelica Miglino
• Grantee: Igor Smirnow Cordeiro
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Chronic skin lesions caused by different etiologies, including different degrees of burn are commonly treated using skin grafts. These can be autologous (where the donor and recipient's own) or allogeneic (where the graft is obtained from other individuals normally cadavers). However, it is common to lack of sufficient tissue to meet all the demand so that alternative therapies become necessary. Among these, biological acellular biomaterials obtained from the decellularization tissue, have been considered an important option. However, the application of this technology for skin replacement requires complete characterization of this in order to guide what the best tissue to be decellularized. For this purpose, Wistar rats will be have the dermis decellularized and morphologically evaluated by histological techniques, electron microscopy and protein analysis. (AU)