Introduction: The melanin-concentrating hormone (MCH) is an important neuropeptide, initially described as an orexigenic factor but associated with a lot of physiological actions, especially in motivated behaviors. Only during lactation it is possible to detect the messenger RNA expression of the prepro-MCH (ppMCH mRNA) and immunoreactivity to MCH in the medial preoptic area (MPOA), a crucial region for maternal behavior expression. Probing the relationship between MCH during the lactation period and peripheral tissues, our group has demonstrated that the dam's mammary gland represents a novel site of MCH receptor 1 (MCHR1) mRNA expression. Therefore, MCH might have a tight relation with peripheral organs related to reproductive system. Aim: Investigate the presence of MCHR1 in the mammary gland, uterus and ovaries of lactating and non-lactating female mice, using the brain as a reference tissue and MCHR1 knockout mice as negative control for all techniques performed. Materials and Methods: The mammary glands, uteri and ovaries of wild type and MCHR1-KO mice in different phases (diestrus, 5th, 12th, 19th days of lactation) will be harvested and processed for alkaline-phosphatase immunohistochemistry.
News published in Agência FAPESP Newsletter about the scholarship:
PACHECO FERREIRA, JOZELIA GOMES;
BITTENCOURT, JACKSON CIONI;
Melanin-concentrating hormone and sleep.
CURRENT OPINION IN NEUROBIOLOGY,
Web of Science Citations: 9.
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