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Characterization of the melanin-concentrating hormone receptor 1 (MCHR1) in the brain, mammary gland, uterus and ovaries in wild type and MCHR1 knockout mice

Grant number: 16/19845-9
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): January 10, 2017
Effective date (End): April 09, 2017
Field of knowledge:Biological Sciences - Morphology
Principal Investigator:Jackson Cioni Bittencourt
Grantee:Jozélia Gomes Pacheco Ferreira
Supervisor abroad: Antoine Adamantidis
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : University of Bern, Switzerland  
Associated to the scholarship:15/05990-4 - The melanin-concentrating hormone [MCH] peptidergic system: neurogenesis and lactation, BP.PD


Introduction: The melanin-concentrating hormone (MCH) is an important neuropeptide, initially described as an orexigenic factor but associated with a lot of physiological actions, especially in motivated behaviors. Only during lactation it is possible to detect the messenger RNA expression of the prepro-MCH (ppMCH mRNA) and immunoreactivity to MCH in the medial preoptic area (MPOA), a crucial region for maternal behavior expression. Probing the relationship between MCH during the lactation period and peripheral tissues, our group has demonstrated that the dam's mammary gland represents a novel site of MCH receptor 1 (MCHR1) mRNA expression. Therefore, MCH might have a tight relation with peripheral organs related to reproductive system. Aim: Investigate the presence of MCHR1 in the mammary gland, uterus and ovaries of lactating and non-lactating female mice, using the brain as a reference tissue and MCHR1 knockout mice as negative control for all techniques performed. Materials and Methods: The mammary glands, uteri and ovaries of wild type and MCHR1-KO mice in different phases (diestrus, 5th, 12th, 19th days of lactation) will be harvested and processed for alkaline-phosphatase immunohistochemistry.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PACHECO FERREIRA, JOZELIA GOMES; GOMES DUARTE, JESSICA CATHARINE; DINIZ, GIOVANNE BARONI; BITTENCOURT, JACKSON CIONI. Litter size determines the number of melanin-concentrating hormone neurons in the medial preoptic area of Sprague Dawley lactating dams. Physiology & Behavior, v. 181, p. 75-79, NOV 1 2017. Web of Science Citations: 2.
PACHECO FERREIRA, JOZELIA GOMES; BITTENCOURT, JACKSON CIONI; ADAMANTIDIS, ANTOINE. Melanin-concentrating hormone and sleep. CURRENT OPINION IN NEUROBIOLOGY, v. 44, p. 152-158, JUN 2017. Web of Science Citations: 7.

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