Aluminum tolerance mechanisms in Styrax camporum involves organic acids secretion?

• Grant number: 16/14216-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2017
• End date: February 28, 2018
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Gustavo Habermann
• Grantee: Brenda Mistral de Oliveira Carvalho
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

Edaphic aluminum (Al) decreases the growth of most plants cultivated in acidic soils. However, some woody species from the Cerrado vegetation accumulate Al in leaves without apparent damage to their metabolism. The reasons why these plants are not sensitive to Al in acidic soils are still unknown. The exudation of organic acids (OAs) from root tips seems to be one of the major Al resistance mechanisms found in tolerant crop species. OAs bind to the Al present in the rhizosphere, forming a stable and non-toxic complex. In addition, studies with several woody plants show that OAs can chelate intracellular Al, allowing Al accumulation as non-toxic complexes. However, there is no evidence of synthesis and secretion of OAs in native woody plants the Cerrado vegetation. Here we chose Styrax camporum Pohl (Styracaceae), a species of natural occurrence in Cerrado areas, which accumulates approximately 1000 mg Al per kg of dry leaf. Young plants will be subjected to three Al concentrations (0, 740 and 1480 micromoles). We will evaluate the OAs in the root tips and in the nutrient solutions after 1h, 12h, 24h, 168 and 360h. We expect that plants exposed to 1480 microM Al will synthesize and secrete more OAs in relation to those exposed to 740 and 0 microM Al. Then, we will determine when the biosynthesis and secretion of AOs start. Data will be submitted to an analysis of variance (One-way ANOVA) and the concentrations of each OA will be tested for each time (1h, 12h, 24h, 168 and 360h). The Shapiro-Wilk test will be used to check for the normality of data. Mean values will be compared by Tukey test (p < 0.05%). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA CARVALHO BITTENCOURT, BRENDA MISTRAL; ZANAO FILHO, SEBASTIAO; HABERMANN, GUSTAVO. Method to quantify aluminum-induced organic acids secretion by roots of plants in nutrient solution using GC-MS. THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY, v. 32, n. 2, p. 121-131, JUN 2020. (16/14216-3)

SILVA, CAROLINA M. S.; CAVALHEIRO, MARIANA F.; BRESSAN, ANNA C. G.; CARVALHO, BRENDA M. O.; BANHOS, OTAVIA F. A. A.; PURGATTO, EDUARDO; HARAKAVA, RICARDO; TANAKA, FRANCISCO A. O.; HABERMANN, GUSTAVO. Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia. Plant Growth Regulation, v. 87, n. 1, p. 123-137, JAN 2019. (16/14216-3, 13/11370-3, 13/07914-8, 14/14386-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARVALHO, Brenda Mistral de Oliveira. The mechanisms of aluminum tolerance in Styrax camporum involve synthesis and secretion of organic acids?. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.