Study of leucotyrene participation on foreign body chronic inflammatory response in Nile tilapias, treated with Zafirlucaste

• Grant number: 16/23103-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2017
• End date: December 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Marco Antonio de Andrade Belo
• Grantee: Susana Luporini de Oliveira
• Host Institution: Universidade Brasil. Campus Descalvado. Descalvado , SP, Brazil

Abstract

Based on the importance of fish farming for the Brazilian economy, associated with the need for technical sanitary development for fish farming, this research aims to study and identify important aspects involved in the pathophysiology of foreign body chronic inflammatory reaction in teleostean fish treated with zafirlucast, through the evaluation of the anti-inflammatory activity. Treatment with 500 ¼g of zafirlukast / kg of live weight, administered orally in the diet, on the kinetics of migration and formation of giant cells in the chronic inflammatory response of tilapia. To this end, 72 tilapias (Oreochromis niloticus), young, packed in 9 aquariums, with a capacity of 100 L of water, will be used, supplied with running water devoid of chlorine, from artesian well with flow rate of 1 L / min. The following treatments were used: T0 (control), T1 (prolonged treatment with Zafirlukast) and T2 (treatment with zafirlukast after inflammatory stimuli). Eight animals will be evaluated per treatment in three periods, ie: two, four and eight days post-implant of the glass coverslips in the subcutaneous tissue to collect blood samples to determine the blood count and the glass coverslips to evaluate the kinetics of accumulation In the inflamed focus after staining of Hematoxylin and Eosin (HE). The correlation between the hematological findings and the cellular count in the inflamed focus will allow the investigation of the participation of leukotrienes in the pathophysiology of the chronic foreign body type inflammatory reaction. In addition to promoting technical-scientific knowledge in aquaculture, the findings of this research may represent important advances in the determination of alternative experimental models for the study of lipoxygenase participation in foreign body type inflammation. (AU)