Blood oxygenation analysis in tumors using photoacoustic imaging

• Grant number: 16/22374-8
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2017
• Effective date (End): March 31, 2021
• Field of knowledge: Engineering - Biomedical Engineering - Medical Engineering
• Principal researcher: Theo Zeferino Pavan
• Grantee: João Henrique Uliana
• Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 13/18854-6 - Photoacoustic and ultrasound imaging applied to tissue characterization, AP.JP

Abstract

Photoacoustic imaging consists in light absorption by a sample generating acoustic waves due to photoacoustic effect. If temporal stress confinement is satisfied a broadband pressure wave will be generated and photoacoustic images can be acquired using a clinical ultrasound machine. Photoacoustic imaging combines contrast of optical images and spatial resolution of ultrasonic images. Due to the capacity of encoding optical proprieties distribution in acoustic waves, this image technique can be used to imaging blood vessels to diagnostics and monitoring of pathologies that change vasculature. Hemoglobin is an endogen chromophore that highly absorbs light, allowing photoacoustic imaging of vasculature. The specific optical absorption of hemoglobin is influenced by oxygenation and present absorption peak in 850 nm for oxy-hemoglobin and 750 nm for deoxy-hemoglobin. Thus, photoacoustic images generated using a LASER with different wave lengths can use this contrast mechanism to investigate oxygen heterogeneity in tumors. Other studies have shown increase in oxygenation in tumor after mild hyperthermia (heating the tissue to around 40°C) due to enhancement in perfusion and decrease of oxygen consumption. Mild hyperthermia in tumoral region can reduce the amount of hypoxic cells, increasing radiosensitivity and improving radiotherapy eficience. Therefore, it is proposed photoacoustic images to evaluate tumor oxygenation in an animal murine model. This evaluation will also be performed in tumors exposed to mild hyperthermia using high intensity focal ultrasound.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ULIANA, JOAO H.; SAMPAIO, DIEGO R. T.; FERNANDES, GUILHERME S. P.; BRASSESCO, MARIA S.; NOGUEIRA-BARBOSA, MARCELLO H.; CARNEIRO, ANTONIO A. O.; PAVAN, THEO Z.. Multiangle Long-Axis Lateral Illumination Photoacoustic Imaging Using Linear Array Transducer. SENSORS, v. 20, n. 14, JUL 2020. (17/14482-8, 16/22374-8, 13/18854-6, 15/05684-0)

AMADO, ANDRE MIELE; ULIANA, JOAO HENRIQUE; PAVAN, THEO ZEFERINO; BORISSEVITCH, IOURI. Effect of metallization on porphyrin photoacoustic response. Chemical Physics Letters, v. 738, JAN 2020. (16/22374-8, 13/18854-6)

CABRELLI, LUCIANA C.; ULIANA, JOAO H.; DA CRUZ JUNIOR, LUISMAR B.; BACHMANN, LUCIANO; CARNEIRO, ANTONIO A. O.; PAVAN, THEO Z.. Glycerol-in-SEBS gel as a material to manufacture stable wall-less vascular phantom for ultrasound and photoacoustic imaging. BIOMEDICAL PHYSICS & ENGINEERING EXPRESS, v. 7, n. 6, NOV 2021. (13/18854-6, 16/22374-8)

HADADIAN, YASER; ULIANA, JOAO H.; CARNEIRO, ANTONIO A. O.; PAVAN, THEO Z.. A Novel Theranostic Platform: Integration of Magnetomotive and Thermal Ultrasound Imaging With Magnetic Hyperthermia. IEEE Transactions on Biomedical Engineering, v. 68, n. 1, p. 68-77, JAN 2021. (16/22374-8, 13/18854-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ULIANA, João Henrique. Systems of image and photoacoustic spectroscopy. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.