Development of microfluidic paper-based analytical device for clinical interest analysis with fluorescence detection

• Grant number: 16/20847-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2017
• End date: February 29, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Helena Redigolo Pezza
• Grantee: Eduardo Luiz Rossini
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 18/05993-1 - Paper-spray mass spectrometry as a tool for doping detection, BE.EP.DR

Abstract

Nanotechnology is a multidisciplinary science that includes scientific and technological activities in the nanometer scale, which includes materials with length between 1 and 100 nm in at least one dimension. Quantum dots are nanoparticles capable to emitting light in the visible region, when excited with electromagnetic radiation of higher energy than the emitted. Quantum dots have wide applicability, more specifically carbon quantum dots (C-dots), which besides the traditional inorganic QDs properties, such as luminescence dependent of the size and wavelength, photodegradation resistance and easy bioconjugation, the C-dots have no intrinsic toxicity, which facilitates application in biological samples, and they are consistent with the philosophy of Green Chemistry. The biocompatibility of C-dots is of great interest for application in clinical analysis, as the dosage of various analytes like glucose, uric acid, creatinine, lactate, among others, provide important information such as renal function, cardiac disease and diabetes. In addition, with the use of microfluidic devices on paper are developed fast test, accurate, easy to use, sensitive, using reagent and sample volumes reduced and deliverable to those who need them, which is consistent with that proposed by the World Health Organization. In this way, the development of µPAD for simultaneous determination of clinical interest compounds using the same fluorescent reagent consisting of carbon quantum dots will be studied.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROSSINI, EDUARDO LUIZ; MILANI, MARIA IZABEL; PEZZA, HELENA REDIGOLO. Green synthesis of fluorescent carbon dots for determination of glucose in biofluids using a paper platform. Talanta, v. 201, p. 503-510, AUG 15 2019. (15/21733-1, 16/20847-6)

ROSSINI, EDUARDO LUIZ; MILANI, MARIA IZABEL; LIMA, LILIANE SPAZZAPAM; PEZZA, HELENA REDIGOLO. Paper microfluidic device using carbon dots to detect glucose and lactate in saliva samples. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 248, MAR 5 2021. (15/21733-1, 16/20847-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROSSINI, Eduardo Luiz. Analysis in paper devices: synthesis of carbon dot for determination of clinical interesting compounds and paper-spray ionization for direct detection of doping in sport. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.