| Grant number: | 16/25907-7 |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| Start date: | March 01, 2017 |
| End date: | February 28, 2018 |
| Field of knowledge: | Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms |
| Principal Investigator: | Mario Henrique de Barros |
| Grantee: | Maria Antônia Kfouri Martins Soares |
| Host Institution: | Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated research grant: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID |
Abstract mRNA translation requires the correct association of the tRNA with the cognate aminoacid. This process is carried by a specific aminoacyl-tRNA synthase. Some bacteria and organelles do not have the complete of aminoacyl tRNA synthases and extra transamidation reactions are therefore required for the aminoacid inespecifically associated to the tRNA. For instance this process occurs in the maturation of Glu-tRNAQ into Gln-tRNAQ.Bacterial GatCAB amidotransferases are responsible for the transamidation of mischarged glutamyl-tRNAGln into glutaminyl-tRNAGln. Mitochondria also have a multienzymatic complex necessary for the transamidation of glutamyl-tRNAGln. Gtf1p, Qrs1p and Pet112p are considered constituents of mitochondrial GatFAB amidotransferase. Our aim here is to identify the human gene ortologus to yeast GTF1 through functional complementation assays using human cDNA libraries . We also intend to proceed in the caracterization of MSC6 , a qrs1 multicopy supressor, studying its involvment in mitochondrial translation. (AU) | |
| News published in Agência FAPESP Newsletter about the scholarship: | |
| More itemsLess items | |
| TITULO | |
| Articles published in other media outlets ( ): | |
| More itemsLess items | |
| VEICULO: TITULO (DATA) | |
| VEICULO: TITULO (DATA) | |