Characterization of genotypic variation and agronomic biofortification of cowpea plants with selenium to improve nutritional quality and low phytate content in grains

• Grant number: 16/19773-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2017
• End date: February 28, 2019
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: André Rodrigues dos Reis
• Grantee: Vinicius Martins Silva
• Host Institution: Faculdade de Ciências e Engenharia. Universidade Estadual Paulista (UNESP). Campus de Tupã. Tupã , SP, Brazil
• Associated scholarship(s): 17/21950-8 - Evaluation of phytate concentration in cowpea: genotypic variation, application of sources and rates of selenium, application of zinc and selenium sulfur interaction, BE.EP.MS

Abstract

There are conclusive evidences of selenium (Se) deficiency in Brazilian soils, which can reflect on Se deficiency in plants, animals and humans. Modern cultivars show low concentration of micronutrient in grains, therefore, is needed to increase Se concentration in edible parts of plants. This study aims to evaluate the influence of Se on yield and nutritional quality of cowpea beans. In addition, the establishment of optimal levels of Se application in Brazilian field conditions will be performed. To achieve these purposes, the following experiments will be conducted. Experiment 1: Based on our former studies, the characterization of genotypic variation on Se uptake by cowpea plants and accumulation of Se in grains will be performed. In this study, 30 genotypes of cowpea will be cultivated in presence and absence of Se (0 and 0.3 mg kg-1). The grains will be collected for nutritional analysis. Experiment 2: The agronomic biofortification will be evaluated by application of 2 sources of Se (selenate and selenite) via application of 7 levels of Se (0; 2,5; 5,0; 7,5; 10,0; 20,0; e 40,0 g ha-1) applied on the soil. At the end of experiments 1 and 2, the nutrient concentration, Se bioavailability, aminoacids, protein, sugar, phytate, myo-inositol (IP5 and IP6) will be analyzed. The better understanding of key factors involved on genotypic variation and selenium accumulation in plants in response to fertilizer, can provide powerful information for plant breeding, mineral nutrition, fertilization, and for production of better nutritional quality food. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, VINICIUS M.; BOLETA, EDUARDO H. M.; MARTINS, JULIANA T.; DOS SANTOS, FLAVIA L. M.; DA ROCHA SILVA, ANNE C.; ALCOCK, THOMAS D.; WILSON, LOLITA; DE SA, MARCO E.; YOUNG, SCOTT D.; BROADLEY, MARTIN R.; et al. Agronomic biofortification of cowpea with selenium: effects of selenate and selenite applications on selenium and phytate concentrations in seeds. Journal of the Science of Food and Agriculture, v. 99, n. 13, JULY 2019. (16/19773-8, 17/21950-8)

SILVA, VINICIUS MARTINS; MARCANDALLI BOLETA, EDUARDO HENRIQUE; DANTAS BERETA LANZA, MARIA GABRIELA; LAVRES, JOSE; MARTINS, JULIANA TRINDADE; SANTOS, ELCIO FERREIRA; MENDES DOS SANTOS, FLAYIA LOURENCO; PUTTI, FERNANDO FERRARI; FURLANI JUNIOR, ENES; WHITE, PHILIP J.; et al. Physiological, biochemical, and ultrastructural characterization of selenium toxicity in cowpea plants. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 150, p. 172-182, JUN 2018. (16/19773-8, 15/19121-8)

SILVA, VINICIUS MARTINS; PUTTI, FERNANDO FERRARI; WHITE, PHILIP J.; DOS REIS, ANDRE RODRIGUES. hytic acid accumulation in plants: Biosynthesis pathway regulation and role in human die. Plant Physiology and Biochemistry, v. 164, p. 132-146, JUL 2021. (16/19773-8, 20/12218-4, 18/18936-6)

SILVA, VINICIUS MARTINS; NARDELI, ANA JULIA; MENDES, NANDHARA ANGELICA CARVALHO; GABRIEL, LUIS ROBERTO ALMEIDA; GABRIEL, CAMILA PIRES CRESMASCO; DOS REIS, ANDRE RODRIGUES. Does Selenium Application Increase the Yield of Cowpea Plants? Evidence from 29 Genotypes on Ureides and Sugar Index Affecting the Yield. JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, v. N/A, p. 10-pg., 2023-08-29. (16/19773-8, 17/21950-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Vinicius Martins. Characterization of genotype variation and agronomic biofortification of cowpea with selenium: impacts on phytic acid and nutritional quality of grains. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Engenharia. Ilha Solteira Ilha Solteira.