Electroretinography (ERG) is a non-invasive procedure which allows recording of electrical activity of the retina. It has been used to evaluate the different cellular circuits involved in visual processing at the retinal level, and has been used to elucidate the pathophysiological mechanisms in glaucoma disease. The aim of this proposal is to use ERG-based techniques to evaluate (1) the extent of the involvement of L- and M-cone contributions to the ERG in glaucoma and controls, and (2) the influence of retinal position on L- and M-driven ERG and those reflecting parvo and magnocellular activity in glaucoma pathophysiology. Participants will be recruited from the 'Glaucoma Registry' of the Department of Ophthalmology at the University Hospital Erlangen, and we will compare the results obtained from glaucoma patients with age-matched healthy controls. L- and M-cone isolating sine wave stimuli will be created with a four- primary LED stimulator using triple silent substitution paradigms. Spatially restricted stimuli of 10° to 20° diameter will be positioned at different locations of the retina and the responses to L- and M-cone isolating stimuli will be measured at two temporal frequencies: 12 Hz (to study parvocellular activity) and 36 Hz (for magnocellular activity). Our results can help to elucidate which (L or M-driven) ERGs reflect the activity of magno and parvocellular pathways in glaucoma. The results will also provide a better understanding of the spatial distribution of ERG signals, allowing the creation of a functional retina map of in healthy and glaucoma patients. The proposed approach might be an objective and sensitive method for the early diagnosis of glaucoma.
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