Biodegradable and pH-responsive polymersomes for gene and protein delivery

• Grant number: 16/23844-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2017
• End date: February 28, 2021
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Fernando Carlos Giacomelli
• Grantee: Lindomar Jose Calumby Albuquerque
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated scholarship(s): 17/11261-0 - Synthesis of PHPMA-b-PDPA, PEO-b-PDPA and RGD-PHPMA-b-PDPA conjugates with further engineering of pH-responsive polymersomes towards the encapsulation and delivery of hydrophilic biomacromolecules, BE.EP.DR

Abstract

The project focuses on the manufacturing of polymeric vesicles (polymersomes) for gene and protein encapsulation and delivery. The first step aims the production and structural characterization of assemblies produced from the block copolymers PEO45-b-PCL118, PEO45-b-PLA174, PHPMAm-b-PDPAn and PEOm-b-PDPAn. The synthesis of the PDPA-based block copolymers is intended to be performed at the Centre of BioMedical Polymers (Czech Republic) during a BEPE internship. The structural characterization will be performed essentially via scattering techniques (DLS, SLS, ELS, SAXS) and imaging (TEM). Subsequently, the encapsulation and release of biomacromolecules (proteins and nucleic acid) will be investigated and further, interaction of the polymeric vesicles with biological system (cells) will be evaluated via MTT cytotoxicity, cellular uptake and intracellular release experiments. Similar investigations will be performed by using the block copolymer functionalized with the peptide sequence RGD (RGD-PHPMAm-b-PDPAn). (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES QUINTINO CORREA, RAISSA LOHANNA; DOS SANTOS, RENAN; CALUMBY ALBUQUERQUE, LINDOMAR JOSE; BARROS DE ARAUJO, GABRIEL LIMA; CHANTE EDWARDS-GAYLE, CHARLOTTE JENNIFER; FERREIRA, FABIO FURLAN; COSTA, FANNY NASCIMENTO. Ciprofibrate-Loaded Nanoparticles Prepared by Nanoprecipitation: Synthesis, Characterization, and Drug Release. POLYMERS, v. 13, n. 18, SEP 2021. (16/23844-8)

DE OLIVEIRA, FERNANDO A.; ALBUQUERQUE, LINDOMAR J. C.; DELECOURT, GWENDOLINE; BENNEVAULT, VERONIQUE; GUEGAN, PHILIPPE; GIACOMELLI, FERNANDO C.. Current Designs of Polymeric Platforms Towards the Delivery of Nucleic Acids Inside the Cells with Focus on Polyethylenimine. CURRENT GENE THERAPY, v. 21, n. 5, p. 431-451, 2021. (16/23844-8, 18/11038-2, 19/20470-8, 19/12944-0)

ALBUQUERQUE, LINDOMAR J. C.; ALAVARSE, ALEX C.; CARLAN DA SILVA, MARIA C.; ZILSE, MORGANA S.; BARTH, MAITE T.; BELLETTINI, ISMAEL C.; GIACOMELLI, FERNANDO C.. Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression. Macromolecular Bioscience, v. 18, n. 2, FEB 2018. (16/23844-8, 17/00459-4)

CERNOCH, PETER; JAGER, ALESSANDRO; CERNOCHOVA, ZULFIYA; SINCARI, VLADIMIR; ALBUQUERQUE, LINDOMAR J. C.; KONEFAL, RAFAL; PAVLOVA, EWA; GIACOMELLI, FERNANDO C.; JAGER, ELIEZER. Engineering of pH-triggered nanoplatforms based on novel poly(2-methyl-2-oxazoline)-b-poly[2-(diisopropylamino)ethyl methacrylate] diblock copolymers with tunable morphologies for biomedical applications. Polymer Chemistry, v. 12, n. 19, p. 2868-2880, MAY 21 2021. (19/06634-8, 16/23844-8, 17/11261-0)

BATISTA, CARIN C. S.; ALBUQUERQUE, LINDOMAR J. C.; DE ARAUJO, IRIS; ALBUQUERQUE, BRUNNO L.; DA SILVA, FERNANDA D.; GIACOMELLI, FERNANDO C.. Antimicrobial activity of nano-sized silver colloids stabilized by nitrogen-containing polymers: the key influence of the polymer capping. RSC ADVANCES, v. 8, n. 20, p. 10873-10882, 2018. (17/00459-4, 16/23844-8)

ALBUQUERQUE, LINDOMAR J. C.; SINCARI, VLADIMIR; JAGER, ALESSANDRO; KUCKA, JAN; HUMAJOVA, JANA; PANKRAC, JAN; PARAL, PETR; HEIZER, TOMAS; JANOUSKOVA, OLGA; DAVIDOVICH, IRINA; et al. pH-responsive polymersome-mediated delivery of doxorubicin into tumor sites enhances the therapeutic efficacy and reduces cardiotoxic effects. JOURNAL OF CONTROLLED RELEASE, v. 332, p. 529-538, APR 10 2021. (16/23844-8)

DE OLIVEIRA, FERNANDO A.; BATISTA, CARIN C. S.; ALBUQUERQUE, LINDOMAR J. C.; CERNOCH, PETER; STEINHART, MILOS; SINCARI, VLADIMIR; JAGER, ALESSANDRO; JAGER, ELIEZER; GIACOMELLI, FERNANDO C.. Tuning the morphology of block copolymer-based pH-triggered nanoplatforms as driven by changes in molecular weight and protocol of manufacturing. Journal of Colloid and Interface Science, v. 635, p. 11-pg., 2023-04-01. (17/24603-7, 16/23844-8, 19/12944-0, 19/06634-8, 21/12071-6)