Effects of methimazole-induced thyroid hormone insufficiency on zebrafish (Danio rerio) spermatogenesis

• Grant number: 17/09218-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: August 30, 2017
• End date: December 14, 2017
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Rafael Henrique Nóbrega
• Grantee: Aldo Tovo Neto
• Supervisor: Hamid Reza Habibi
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: University of Calgary, Canada
• Associated to the scholarship: 14/16280-5 - Molecular and endocrine control involved on gonadal differentiation of zebrafish (Danio rerio), BP.DR

Abstract

The role of thyroid hormone on testicular development is well described in mammals, however in fish is poorly explored. Considering that in mammals most of the experimental studies to date are focused on thyroid hormone effects on the developing testes, only limited data are available on adult testis and spermatogenesis. Therefore, the aim of this study is to evaluate the effects of thyroid hormone on zebrafish testis function and spermatogenesis. First, males will be exposed with 1 mM methimazole, a thyroid hormone disruptor for 21 days. Next, testes will be dissected out and cultured using an ex vivo organ culture system to study the effects of T3 on androgen release, spermatogenesis, gene expression and caspase activity. Spermatogenesis will be quantified evaluating cyst frequency and germ cell proliferation. The results of this study will provide contributions to teleost reproductive physiology and to comparative endocrinology. We expect to clarify the mechanisms how thyroid hormone act in the spermatogenesis and testis function. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOVO-NETO, ALDO; RODRIGUES, MAIRA DA SILVA; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Thyroid hormone actions on male reproductive system of teleost fish. General and Comparative Endocrinology, v. 265, n. SI, p. 230-236, SEP 1 2018. (14/16280-5, 14/07620-7, 17/09218-0, 17/15793-7)

FALLAH, HAMIDEH P.; TOVO-NETO, ALDO; YEUNG, EDWARD C.; NOBREGA, RAFAEL H.; HABIBI, HAMID R.. Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone. Molecular and Cellular Endocrinology, v. 492, JUL 15 2019. (14/07620-7, 17/09218-0, 14/16280-5)

TOVO-NETO, ALDO; MARTINEZ, EMANUEL R. M.; MELO, ALINE G.; DORETTO, LUCAS B.; BUTZGE, ARNO J.; RODRIGUES, MAIRA S.; NAKAJIMA, RAFAEL T.; HABIBI, HAMID R.; NOBREGA, RAFAEL H.. Cortisol Directly Stimulates Spermatogonial Differentiation, Meiosis, and Spermiogenesis in Zebrafish (Danio rerio) Testicular Explants. BIOMOLECULES, v. 10, n. 3, MAR 2020. (14/07620-7, 14/16280-5, 13/22778-3, 17/15793-7, 16/12101-4, 15/12104-0, 17/09218-0)

TOVO-NETO, ALDO; RODRIGUES, MAIRA DA SILVA; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Thyroid hormone actions on male reproductive system of teleost fish. General and Comparative Endocrinology, v. 265, p. 7-pg., 2018-09-01. (17/09218-0, 14/07620-7, 14/16280-5, 17/15793-7)