Effects of hypothyroidism on testicular function of Danio rerio and relation between thyroid hormones and follicle stimulating hormone (Fsh)

• Grant number: 17/15793-7
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): November 01, 2017
• Effective date (End): July 31, 2019
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
• Principal Investigator: Rafael Henrique Nóbrega
• Grantee: Maira da Silva Rodrigues
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 14/07620-7 - Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species, AP.JP
• Associated scholarship(s): 18/15319-6 - Cross-talk between gonadal gonadotropin-inhibitory hormone (GnIH) and thyroid hormones (THs) in the hypothalamic-pituitary-peripheral axis of zebrafish (Danio rerio), BE.EP.MS

Abstract

Thyroid hormones exert several roles on vertebrate homeostasis, acting in many physiological processes, such as growth regulation, behavior, stress and also in the reproductive system. In mammals, the role of thyroid hormone (T3) in reproduction has been well established, however, when regarding fish endocrinology the effects of this hormone on fish reproduction specifically on spermatogenesis are still unknown. In this context, recent studies have shown that T3 enhanced the action of gonadotropin (Fsh), a potent steroidogenic hormone in fish. Therefore, the current research aims to elucidate the effects the hypothyroidism effects on fish testis function and also unveil the role between T3 and Fsh. For this purpose, fish will be exposed to methimazole (1 mM), a compound that blocks thyroid hormone production, for 21 days. After exposure, testis will be collected for morphological, morphometrical, steroid release, gene expression and apoptosis analysis. Moreover, testis will be in vitro cultivated with Fsh in the presence or absence of T3 and subjected to same analysis. At the end, this research will provide support for a better understanding of the endocrine and molecular control involved in fish spermatogenesis. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOVO-NETO, ALDO; RODRIGUES, MAIRA DA SILVA; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Thyroid hormone actions on male reproductive system of teleost fish. General and Comparative Endocrinology, v. 265, n. SI, p. 230-236, SEP 1 2018. (14/16280-5, 14/07620-7, 17/09218-0, 17/15793-7)

DE SIQUEIRA-SILVA, DIOGENES HENRIQUE; RODRIGUES, MAIRA DA SILVA; NOBREGA, RAFAEL HENRIQUE. Testis structure, spermatogonial niche and Sertoli cell efficiency in Neotropical fish. General and Comparative Endocrinology, v. 273, n. SI, p. 218-226, MAR 1 2019. (14/07620-7, 17/15793-7)

FALLAH, HAMIDEH P.; RODRIGUES, MAIRA S.; ZANARDINI, MAYA; NOBREGA, RAFAEL H.; HABIBI, HAMID R.. Effects of gonadotropin-inhibitory hormone on early and late stages of spermatogenesis in ex-vivo culture of zebrafish testis. Molecular and Cellular Endocrinology, v. 520, JAN 15 2021. (17/15793-7, 18/15319-6)

FALLAH, HAMIDEH P.; RODRIGUES, MAIRA S.; CORCHUELO, SHERYLL; NOBREGA, RAFAEL H.; HABIBI, HAMID R.. Role of GnRH Isoforms in Paracrine/Autocrine Control of Zebrafish (Danio rerio) Spermatogenesis. Endocrinology, v. 161, n. 2, FEB 2020. (17/15793-7, 18/15319-6)

TOVO-NETO, ALDO; MARTINEZ, EMANUEL R. M.; MELO, ALINE G.; DORETTO, LUCAS B.; BUTZGE, ARNO J.; RODRIGUES, MAIRA S.; NAKAJIMA, RAFAEL T.; HABIBI, HAMID R.; NOBREGA, RAFAEL H.. Cortisol Directly Stimulates Spermatogonial Differentiation, Meiosis, and Spermiogenesis in Zebrafish (Danio rerio) Testicular Explants. BIOMOLECULES, v. 10, n. 3, MAR 2020. (14/07620-7, 14/16280-5, 13/22778-3, 17/15793-7, 16/12101-4, 15/12104-0, 17/09218-0)

RODRIGUES, MAIRA S.; FALLAH, HAMIDEH P.; ZANARDINI, MAYA; MALAFAIA, GUILHERME; HABIBI, HAMID R.; NOBREGA, RAFAEL H.. Interaction between thyroid hormones and gonadotropin inhibitory hormone in ex vivo culture of zebrafish testis: An approach to study multifactorial control of spermatogenesis. Molecular and Cellular Endocrinology, v. 532, JUL 15 2021. (17/15793-7, 18/15319-6, 14/07620-7)

RODRIGUES, MAIRA S.; TOVO-NETO, ALDO; ROSA, IVANA F.; DORETTO, LUCAS B.; FALLAH, HAMIDEH P.; HABIBI, HAMID R.; NOBREGA, RAFAEL H.. Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and-Gonadal Axes in Zebrafish. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 10, p. 16-pg., 2022-05-12. (17/15793-7, 14/07620-7, 18/15319-6, 20/03569-8)

TOVO-NETO, ALDO; RODRIGUES, MAIRA DA SILVA; HABIBI, HAMID R.; NOBREGA, RAFAEL HENRIQUE. Thyroid hormone actions on male reproductive system of teleost fish. General and Comparative Endocrinology, v. 265, p. 7-pg., 2018-09-01. (17/09218-0, 14/07620-7, 14/16280-5, 17/15793-7)

DE SIQUEIRA-SILVA, DIOGENES HENRIQUE; RODRIGUES, MAIRA DA SILVA; NOBREGA, RAFAEL HENRIQUE. Testis structure, spermatogonial niche and Sertoli cell efficiency in Neotropical fish. General and Comparative Endocrinology, v. 273, p. 9-pg., 2019-03-01. (14/07620-7, 17/15793-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RODRIGUES, Maira da Silva. Effects of hypothyroidism on the testicular function of Danio rerio and relation between thyroid hormones and hypothalamic-pituitary-testes axis. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.