Fallah, Hamideh P.
[1, 2, 3]
Yeung, Edward C.
Nobrega, Rafael H.
Habibi, Hamid R.
Total Authors: 5
 Univ Calgary, Dept Biol Sci, 2500 Univ Dr NW, Calgary, AB T2N 1N4 - Canada
 Sao Paulo State Univ, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP - Brazil
 Sao Paulo State Univ Unesp, Aquaculture Program, Jaboticabal, SP - Brazil
Total Affiliations: 3
Molecular and Cellular Endocrinology;
JUL 15 2019.
Web of Science Citations:
Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response. (AU)