Development of uniplex and multiplex diagnostic platforms for arbovirus infection using recombinant proteins

• Grant number: 16/23560-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2017
• End date: May 31, 2021
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Luis Carlos de Souza Ferreira
• Grantee: Robert Andreata Santos
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 19/16049-5 - Development of uniplex and multiplex diagnostic platforms for arbovirus infection using recombinant proteins, BE.EP.DR

Abstract

Dengue (DENV), Zika (ZIKV) and Chikungunya (CHIV) are the most evident arboviruses in the current international scenario. These three viruses are transmitted by mosquitoes of the Aedes genus and there are face difficulties regarding the generation of accurate epidemiological data when they co-circulate in the same geographic region, as in Brazil. This fact is ascribed to the high similarity of clinical symptoms of the diseases and the extensive cross-reaction observed in serological tests. The availability of sensitive, specific and low cost serological tests, especially for ZIKV and CHIV, is still limited in the country, thus, the aim of the present project is to develop uniplex (ELISA, IFA) and multiplex (Luminex) serological tests based on the use of recombinant forms of structural and non-structural proteins of DENV, ZIKV and CHIV. The emergence of the zika network during the ZIKV outbreak in São Paulo resulted in a strong effort to develop serological tests with high sensitivity and specificity against DENV and ZIKV. As a consequence, recombinant forms of envelope (E) and non-structural (NS1) protein portions that have low homology between the three viruses will be produced. After the antigenic evaluation, the proteins will be used in serodiagnostic platforms that can be easily translated into products (ELISA and IFA) and implemented in the large scale diagnostic laboratories and health centers of the country. The validations of the specific serological tests generated will be carried out with human and nonhuman primates serum libraries already provided by collaborators. Thus, it is expected that the knowledge generated during this thesis will contribute to the development of different serodiagnostic methods specific for DENV, ZIKV and CHIV. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANDREATA-SANTOS, ROBERT; PEREIRA, SAMUEL SANTOS; PEREIRA, LENNON RAMOS; FELIX, ALVINA CLARA; ROMANO, CAMILA MALTA; DE SOUZA FERREIRA, LUIS CARLOS. Specificity of NS1-based immunochromatographic tests for dengue virus with regard to the Zika virus protein. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 95, p. 276-278, JUN 2020. (16/20045-7, 16/23560-0)

PEREIRA, LENNON RAMOS; DOS SANTOS ALVES, RUBENS PRINCE; SALES, NATIELY SILVA; ANDREATA-SANTOS, ROBERT; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; PEREIRA, SAMUEL SANTOS; CASTRO-AMARANTE, MARIA FERNANDA; RODRIGUES-JESUS, MONICA JOSIANE; DE PINHO FAVARO, MARIANNA TEIXEIRA; CHURA-CHAMBI, ROSA MARIA; et al. Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein. FRONTIERS IN MEDICAL TECHNOLOGY, v. 2, p. 14-pg., 2020-12-03. (14/17595-0, 16/20045-7, 16/23560-0, 18/14459-9, 16/05570-8, 18/08199-4, 17/09661-0, 16/14344-1)

ARAUJO, SERGIO C.; PEREIRA, LENNON R.; ALVES, RUBENS P. S.; ANDREATA-SANTOS, ROBERT; KANNO, ALEX I.; FERREIRA, LUIS CARLOS S.; GONCALVES, VIVIANE M.. Anti-Flavivirus Vaccines: Review of the Present Situation and Perspectives of Subunit Vaccines Produced in Escherichia coli. VACCINES, v. 8, n. 3, SEP 2020. (18/24683-3, 16/20045-7, 16/23560-0, 19/18205-4, 15/02352-7)

VENCESLAU-CARVALHO, ALEXIA ADRIANNE; FAVARO, MARIANNA TEIXEIRA DE PINHO; PEREIRA, LENNON RAMOS; RODRIGUES-JESUS, MONICA JOSIANE; PEREIRA, SAMUEL SANTOS; ANDREATA-SANTOS, ROBERT; ALVES, RUBENS PRINCE DOS SANTOS; CASTRO-AMARANTE, MARIA FERNANDA; RODRIGUES, KARINE BITENCOURT; DA SILVA, JAMILE RAMOS; et al. Nano-multilamellar lipid vesicles loaded with a recombinant form of the chikungunya virus E2 protein improve the induction of virus-neutralizing antibodies. Nanomedicine-Nanotechnology Biology and Medicine, v. 37, OCT 2021. (17/23281-6, 16/19145-7, 16/08204-2, 17/24769-2, 16/20045-7, 16/23560-0)

PRUDENCIO, CARLOS ROBERTO; DA COSTA, VIVALDO GOMES; ROCHA, LETICIA BARBOZA; DA COSTA, HERNAN HERMES MONTEIRO; ORTS, DIEGO JOSE BELATO; SANTOS, FELIPE ROCHA DA SILVA; RAHAL, PAULA; LINO, NIKOLAS ALEXANDER BORSATO; DA CONCEICAO, PAMELA JOYCE PREVIDELLI; BITTAR, CINTIA; et al. Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests. Viruses-Basel, v. 15, n. 3, p. 20-pg., 2023-03-01. (16/20045-7, 16/16188-7, 17/50333-7, 16/23560-0, 15/17178-2, 16/05570-8, 17/24769-2)

ANDREATA-SANTOS, ROBERT; MACHADO, RAFAEL RAHAL GUARAGNA; DOS SANTOS ALVES, RUBENS PRINCE; SALES, NATIELY SILVA; SOARES, CAMILA PEREIRA; RODRIGUES, KARINE BITENCOURT; SILVA, MARIANGELA OLIVEIRA; FAVARO, MARIANNA TEIXEIRA DE PINHO; RODRIGUES-JESUS, MONICA JOSIANE; YAMAMOTO, MARCIO MASSAO; et al. Validation of Serological Methods for COVID-19 and Retrospective Screening of Health Employees and Visitors to the Sao Paulo University Hospital, Brazil. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 8-pg., 2022-06-02. (20/08943-5, 16/20045-7, 21/05661-1, 14/50890-5, 16/23560-0, 15/02352-7, 20/06409-1, 17/24769-2, 16/14344-1, 18/23680-0, 20/10700-3, 18/07629-5, 18/07142-9)

RODRIGUES-JESUS, MONICA JOSIANE; FAVARO, MARIANNA TEIXEIRA DE PINHO; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; DE CASTRO-AMARANTE, MARIA FERNANDA; ALMEIDA, BIANCA DA SILVA; SILVA, MARIANGELA DE OLIVEIRA; ANDREATA-SANTOS, ROBERT; BARBOSA, CECILIA GOMES; BRITO, SAMANTHA CARVALHO MAIA; FREITAS-JUNIOR, LUCIO H.; et al. Nano-multilamellar lipid vesicles promote the induction of SARS-CoV-2 immune responses by a protein-based vaccine formulation. Nanomedicine-Nanotechnology Biology and Medicine, v. 45, p. 11-pg., 2022-08-31. (20/10700-3, 20/08943-5, 16/23560-0, 20/05204-7, 21/05661-1)

ANDREATA-SANTOS, ROBERT; DOS SANTOS ALVES, RUBENS PRINCE; PEREIRA, SARA ARAUJO; PEREIRA, LENNON RAMOS; DE FREITAS, CARLA LONGO; PEREIRA, SAMUEL SANTOS; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; CASTRO-AMARANTE, MARIA FERNANDA; PINHO FAVARO, MARIANNA TEIXEIRA; MATHIAS-SANTOS, CAMILA; et al. Transcutaneous Administration of Dengue Vaccines. Viruses-Basel, v. 12, n. 5, MAY 2020. (16/05570-8, 12/50362-3, 16/20045-7, 14/17595-0, 16/23560-0, 15/02352-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Robert Andreata. Development of uniplex and multiplex diagnostic platforms for arbovirus infection using recombinant proteins.. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.