| Grant number: | 17/01753-3 |
| Support Opportunities: | Scholarships in Brazil - Master |
| Start date: | July 01, 2017 |
| End date: | October 31, 2019 |
| Field of knowledge: | Agronomical Sciences - Agronomy - Crop Science |
| Agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Luciana Lasry Benchimol-Reis |
| Grantee: | Caléo Panhoca de Almeida |
| Host Institution: | Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Campinas , SP, Brazil |
| Associated scholarship(s): | 18/15526-1 - Genome-wide association study for identifying agronomic candidate loci in common bean, BE.EP.MS |
Abstract Among the common bean diseases of the, the angular leaf spot (ALS) cause reduction in the yield and the quality of grain produced, and due to the great variability of this pathogen, a durable resistance of many cultivars was not achieved. A large number of molecular markers were associated with QTLs for resistance to this disease by our group of research. According to studies of our research group, ALS showed quantitative inheritance and several QTLs should be introgressed in order to produce a lasting resistance to different races of the pathogen. The AB-QTL (Advanced backcross QTL Analysis) technique is able to select, introgress and simultaneously map the QTLs, through backcrossing, and therefore the effects (epistatic and pleiotropic) of the combination of different QTLs can be measured, and only the QTLs favorable are transferred, without losing the quality of elite cultivar used as the recurrent parent. While the other methods of assisted selection are based on first performing the mapping of the QTLs for then transferring those that are favorable, the AB-QTL method suggests evaluating simultaneously the families and/or progenies that phenotypically superior together with the introgression of QTLs, reducing the problem of linkage drag and negative interactions. Phenotypic selection eliminates the progenies with lower performance; even those that have favorable QTLs introgressed, but can have their values changed by genetic epistatic, pleiotropic effects or by genotype x environment interactions. The objective of this project is to use the technique of AB-QTL to promote a gain on the genetic resistance to ALS in common bean breeding program of the Agronomic Institute (IAC). The 'IAC Milênio' cultivar (susceptible, recurrent parental) was crossed with the 'AND 277' cultivar (resistant, parental donor) in a backcross program since 2014. Phenotypical selection of plants that have desirable agronomical patterns for common bean was performed in RC2F2 for ´carioca1' type, and evaluation with molecular markers in large scale will occur in F3BC22. In this generation, the QTLs will be mapped using GBS, useful for the detection and selection of a high number of SNP markers, and near isogenic lines (QTL-NILs) will be evaluated. The disease phenotyping will be taken in the greenhouse with repeated trials. It is expected that new cultivars may be recommended for the common bean breeding program of the IAC and that they show effective resistance in harvesting conditions. (AU) | |
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