In vitro study of the modulation of innate and adaptive immunity by dysplastic keratinocytes

• Grant number: 17/01798-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2017
• End date: January 31, 2020
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Andreia Bufalino
• Grantee: Darcy Fernandes
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Squamous cell carcinoma (SCC) accounts for more than 95% of all malignancies involving the oral cavity, and often these tumors are preceded by clinical alterations that have a clear potential for malignant transformation, which are denominated oral potentially malignant disorders (OPMDs). Among these, Oral Leukoplakia (OL) is the most important and has a malignant transformation rate ranging from 0.2% to 17.5%. Another important OPMD is Proliferative Verrucous Leukoplakia (PVL), which presents persistent and progressive malignant behavior, with a malignant transformation rate greater than 70%. Differently from OL, risk factors such as tobacco, alcohol and areca do not seem to be associated with the development of PVL. Additionally, PVL presents inadequate response to all treatment modalities, undergoes rapid spread for multiple oral sites and often presents recurrence. Recent studies and preliminary data obtained by our group suggest that the inflammatory infiltrate associated with the lesions of patients with PVL is related to the etiology and/or distinct clinical behavior from the conventional OL. Thus, this study intends to carry out an analysis to determinate the effect of soluble products and/or direct contact of dysplastic cells on the modulation of innate and adaptive immunity and evaluate whether such modulation confers or not an advantage to the tumor progression. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, DARCY; BARBEIRO, CAMILA DE OLIVEIRA; PALACON, MARIANA PARAVANI; BIANCARDI, MARIEL RUIVO; FERRISSE, TULIO MORANDIN; SILVEIRA, HEITOR ALBERGONI; CASTILHO, ROGERIO MORAES; DE ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE; BUFALINO, ANDREIA. High density of CD8 T cell and immune imbalance of T lymphocytes subsets are associated with proliferative verrucous leukoplakia. IMMUNOLOGY, v. 168, n. 1, p. 14-pg., 2022-09-03. (17/01798-7, 17/01438-0, 13/07276-1, 18/22236-0, 21/01191-0, 18/04954-2, 17/17288-8)

PALACON, MARIANA PARAVANI; BARBEIRO, CAMILA DE OLIVEIRA; FERNANDES, DARCY; BIANCARDI, MARIEL RUIVO; SILVEIRA, HEITOR ALBERGONI; FERRISSE, TULIO MORANDIN; LEON, JORGE ESQUICHE; KUJAN, OMAR; BUFALINO, ANDREIA. Macrophages CD163+and Factor XIIIa plus Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 6, p. 12-pg., 2023-03-01. (18/04954-2, 18/22236-0, 13/07276-1, 21/01191-0, 17/01798-7, 17/17288-8)

BARBEIRO, CAMILA DE OLIVEIRA; FERNANDES, DARCY; PALACON, MARIANA PARAVANI; CASTILHO, ROGERIO MORAES; DE ALMEIDA, LUCIANA YAMAMOTO; BUFALINO, ANDREIA. Inflammatory Cells Can Alter the Levels of H3K9ac and gamma H2AX in Dysplastic Cells and Favor Tumor Phenotype. JOURNAL OF PERSONALIZED MEDICINE, v. 13, n. 4, p. 15-pg., 2023-04-01. (18/04954-2, 22/14672-0, 18/22236-0, 17/01798-7, 17/01438-0)

ARROYO, ESTEBAN; PINEIRO DONIS, SERGIO; CHAMORRO PETRONACCI, CINTIA M.; OLIVEIRA ALVES, MONICA G.; MARICHALAR MENDIA, XABIER; FERNANDES, DARCY; LORENZO POUSO, I, ALEJANDRO; BUFALINO, ANDREIA; BRAVO LOPEZ, SUSANA; PEREZ SAYANS, MARIO. Usefulness of protein-based salivary markers in the diagnosis of oral potentially malignant disorders: A systematic review and meta-analysis. CANCER BIOMARKERS, v. 32, n. 4, p. 411-424, 2021. (17/01438-0, 17/01798-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERNANDES, Darcy. Ex vivo and in vitro study of the modulation of innate and adaptive immunity by dysplastic keratinocytes in oral leukoplakia and proliferative verrucous leukoplakia. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.