Effects of concurrent training on the epigenetic mechanism of DNA methylation

• Grant number: 17/01297-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2017
• End date: September 30, 2019
• Field of knowledge: Health Sciences - Physical Education
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Carlos Ugrinowitsch
• Grantee: Guilherme Defante Telles
• Host Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 18/16513-0 - The effects of concurrent training on the epigenetic mechanism of DNA methylation, BE.EP.MS

Abstract

The Dna Methylation Level responses in myogenic genes related to satellite cells (SCs) activation can be mechanisms that explain the the lower muscle gains with Concurrent Training (CT) compared to Resistance Training (RT) alone, phenomenon known as Interference Effect. Aim: To ascertain and compare the CT-induced and RT-induced changes on DNA methylation levels and gene expression. Methods: 12 untrained young men will participate in a randomized and balanced cross over experimental design. Each volunteer will perform a bout of Resistance exercise (RE), endurance exercise (EE) and concurrent exercise (CE). The RE will be composed by 2 exercises (Leg Press 45 and Leg extension), 5x8-12 maximum repetitions with 90s rest. The EE will be performed on a treadmill, composed by 5 x 4 minutes sprints at 85% of maximum oxygen consumption (VO2max) and 3 minutes rest. The CE will be composed by the same RE protocol followed by EE. Twelve muscle samples will be taken: immediately before, and 3 times after each exercise session. These biopsies time points will be determined by a pilot study. Our hypothesis is that the methylation level of myogenic gene downregulation with CE will be lower than with RE, inducing the Interference Effect. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TELLES, GUILHERME DEFANTE; LIBARDI, CLEITON AUGUSTO; CONCEICAO, MIGUEL SOARES; VECHIN, FELIPE CASSARO; LIXANDRAO, MANOEL EMILIO; LUGNANI DE ANDRADE, ANDRE LUIS; GUEDES, DANIEL NOVAIS; UGRINOWITSCH, CARLOS; CAMERA, DONNY MICHAEL. Time Course of Skeletal Muscle miRNA Expression after Resistance, High-Intensity Interval, and Concurrent Exercise. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 53, n. 8, p. 1708-1718, AUG 2021. (18/12150-0, 18/16513-0, 17/01297-8)

TELLES, GUILHERME DEFANTE; LIBARDI, CLEITON AUGUSTO; CONCEICAO, MIGUEL SOARES; VECHIN, FELIPE CASSARO; LIXANDRAO, MANOEL EMILIO; ROTEA MANGONE, FLAVIA REGINA; PAVANELLI, ANA CAROLINA; NAGAI, MARIA APARECIDA; CAMERA, DONNY MICHAEL; HAWLEY, JOHN A.; et al. Interrelated but Not Time-Aligned Response in Myogenic Regulatory Factors Demethylation and mRNA Expression after Divergent Exercise Bouts. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 55, n. 2, p. 10-pg., 2023-02-01. (18/12150-0, 18/16513-0, 17/01297-8)

TELLES, GUILHERME DEFANTE; LIXANDRAO, MANOEL; CONCEICAO, MIGUEL. Effects of combined exercise training in older adults: a potential relationship between muscle fibre satellite cell function and capillarization. JOURNAL OF PHYSIOLOGY-LONDON, v. 597, n. 8, p. 2-pg., 2019-04-15. (17/01297-8, 18/16513-0, 16/22635-6, 15/19756-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TELLES, Guilherme Defante. Effects of concurrent exercise on the methylation and gene expression levels of the myogenic regulatory factors. 2019. Master's Dissertation - Universidade de São Paulo (USP). Escola de Educação Física e Esportes (EEFE/BT) São Paulo.