Morphological and molecular study of Oomycota species isolated from trout farm (Oncorhynchus mykiss Walbaum) in Campos do Jordão, Sao Paulo state, Brazil

• Grant number: 16/18787-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2017
• End date: December 31, 2019
• Field of knowledge: Biological Sciences - Botany - Plant Taxonomy
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Carmen Lidia Amorim Pires-Zottarelli
• Grantee: Sarah Cristina de Oliveira da Paixão
• Host Institution: Instituto de Botânica. Secretaria de Meio Ambiente, Infraestrutura e Logística (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Rainbow trout (Oncorhynchus mykiss Walbaum) originating in North America and introduced in Brazil in the 40s, is currently an example of success in farming of fish in cold and mountainous regions of the country. However, problems with parasites have caused concern to fish farmers because pathogens affect the quality of fish and interfers in the cultivation system. Saprolegnosis, disease caused by oomycete Saprolegnia, is the most known parasitosis and is mainly present in the eggs of O. mykiss. Representatives of other genus Oomycota phylum are also usually recorded as pathogens such as Achlya, Aphanomyces and Dictyuchus. In view of this, this project was developed in order to evaluate the richness and diversity of the representatives of the phylum Oomycota in trout farms located in Campos do Jordao, at different stages of fish farming. For this, samples will be collected from water supply systems; the breeding tanks, in different stages of development of trout; as well as the water outlet of the tanks. In the laboratory, samples are treated by multiple baiting method with cellulosic and keratinous substrates in order to obtain the largest number of specimens. Fish and symptomatic eggs will be also analyzed through direct sampling of mycelium and subsequent inoculation in culture medium. The species will be identified by morphological and molecular analyzes, documented, described and incorporated in the "Instituto de Botânica" collection (CCIBt and / or Herbarium SP).The sequences obtained will be made available in the GenBank database. This project is First mover in Brazil considering the study of oomycetes diversity in trout farming system as well as the various stages of development of the fish, thus enabling the detection of any pathogen entry points in the system. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMORIM PIRES-ZOTTARELLI, CARMEN LIDIA; COLOMBO, DEBORA R. S.; DA PAIXAO, SARAH C. O.; VENTURA, POLIANA O.; BORO, MARCELA C.; DE JESUS, ANA L.. Aphanomyces brasiliensis sp. nov. (Verrucalvaceae, Saprolegniales): a new species from Brazilian Atlantic Rainforest areas. PHYTOTAXA, v. 415, n. 4, p. 208-216, SEP 2 2019. (16/11146-4, 16/18787-5, 18/05602-2)

DIMA, BALINT; BRANDRUD, TOR ERIK; CORRIOL, GILES; JANSEN, GERRIT MAARTEN; JORDAL, JOHN BJARNE; KHALID, ABDUL N.; LARSSON, ELLEN; LORAS, JOSTEIN; MOROZOVA, V, OLGA; NASEER, AROOJ; et al. Fungal Systematics and Evolution: FUSE 7. SYDOWIA, v. 73, p. 271-340, 2021. (16/18787-5, 16/11146-4, 18/05602-2)