Effect of in vitro lipemia on biochemical parameters of healthy dogs and its comparison with in vivo lipemia

• Grant number: 17/05105-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2017
• End date: July 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Breno Fernando Martins de Almeida
• Grantee: Natália Camila Minucci Bonatto
• Host Institution: Faculdades Integradas de Ourinhos (FIO). Fundação Educacional Miguel Mofarrej. Ourinhos , SP, Brazil

Abstract

Lipemia is among the main causes of rejection of samples in veterinary clinical laboratories, interfering with biochemical determinations by spectrophotometry. However, it is not known adequately if such interferences cause clinical errors in routine canine biochemical determinations, especially important when considering that lipemia can not always be avoided in the veterinary routine. This is the third of a series of studies that will evaluate the effect of lipemia on canine biochemical parameters and the present work will evaluate the effect of lipemia simulated "in vitro" using commercial lipid solution on the routine biochemical determinations of healthy dogs and its comparison to in vivo lipemia. Fifteen healthy dogs will be selected, the peak of postprandial hypertriglyceridemia determined in the first study will be used to induce postprandial in vivo lipemia (Proc. 2017 / 04175-0, under review). Subsequently, non-lipemic samples after 12 h fasting will be added commercial lipid solution simulating triglyceride concentrations similar to the minimum, medium and maximum values obtained in the in vivo study. The biochemical analyzes will be performed in an automated spectrophotometer using a set of commercial reagents. The variables will be tested for normality and will be used t-test or Wilcoxon tests to verify the differences between the groups with the non-lipemic sample. (AU)