|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||September 01, 2017|
|Effective date (End):||July 31, 2019|
|Field of knowledge:||Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding|
|Principal Investigator:||Valquíria Cação Cruz-Polycarpo|
|Home Institution:||Faculdade de Ciências Agrárias e Tecnológicas. Universidade Estadual Paulista (UNESP). Campus de Dracena. Dracena , SP, Brazil|
|Associated scholarship(s):||18/22986-9 - Effect of uncoated and microencapsulated butyric acid on the diets of broilers challenged with Eimeria spp. on the count of intestinal goblet cells, BE.EP.MS|
The aim of this study is to evaluate the effect of free and protected butyric acid, associated or not to replace antibiotic growth promoters on the performance, carbon turnover of intestinal mucosa, histomorphometry and small intestine lesion scores beyond count oocysts from feces of broilers challenged with Eimeria acervulina, E. maxima and E. tenella. For this, 1,200 male broiler chicks of line Cobb® will be used, distributed in a completely randomized design, with five treatments and six replicates. The treatments will consist of: T1 - basal diet; T2 - basal diet + antibiotic; T3 - basal diet + butyric acid; T4 - basal diet + protected butyric acid; T5 - basal diet + butyric acid + protected butyric acid. After accommodation, to evaluate mucosal turnover rate, the birds will receive diets predominantly C3. At 16 days of age all birds of all treatments will be inoculated orally and individually with Eimerias oocysts and, after 21 days, to evaluate the turnover rate after challenge, the birds will receive a predominantly C4 diet. Mucosa samples will be analyzed isotopically by mass spectrometry before and after challenge. Performance data will be collected in the accumulated periods from 1 to 14, 1 to 21 and 1 to 42 days of age. For morphometric analysis of the small intestine, to 14 and 21 days of age, will be collected two segments of three centimeters of the duodenum and the jejunum of six birds per treatment. At 5, 6, 7, 8, 9, 10, 12, and 19 days after inoculation will be evaluated the lesion scores of the small intestine. At 4, 5, 6 and 7 days after inoculation, excreted samples will be collected from each experimental unit for count of oocysts. Data analysis will be performed using the SAS (2012) with a 5% significance criterion, and intestinal mucosa turnover analyzes will be performed using the exponential regression method. The treatments will be analyzed using orthogonal contrasts. In addition, the Tukey test can be used to compare pairs of means.