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Maternal and paternal diet effects on the programming of 21-day old offspring until adulthood

Grant number: 17/09646-1
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): September 01, 2017
Effective date (End): August 31, 2021
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Luciana Pellegrini Pisani
Grantee:Helena de Cássia César Vallera
Home Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

Recently, a high-fat diet has been associated with hypothalamic low-grade inflammation, and dysregulation of energy and metabolic control that can lead to the development of obesity. In this process, the connection between gut and brain seems to be involved in the alterations that leads to the development of chronic diseases. Considering the evidence that an inadequate maternal diet, and more recently a paternal diet, can program the offspring to develop chronic diseases into adulthood, we will assess the effects of maternal and paternal cafeteria diet on the programming of the hypothalamus and colon of 21 and 90-day old offspring. Therefore, we will assess in the 21 and 90-day old offspring from parents who had cafeteria or control diets, the following parameters: serum fatty acids profile; hypothalamic and colonic relative weight; leptin and insulin content in the hypothalamus; cytokines TNF-±, IL-6, IL10 and LPS content in the hypothalamus and colon; TLR4 content and proteins involved in the TLR4 pathway MYD88, NFkB fosforilado (p50 e p65) content in the hypothalamus and colon; ZO-1 content in the hypothalamus and colon; quantification of Lactobacillus and Bifidobacterium DNA in the stools. (AU)