Influence of adquired pellicle and timing of application of the AmF/NaF/SnCl2 solution on its potential to control the progression of human enamel erosion.

• Grant number: 16/08524-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2017
• End date: August 31, 2019
• Field of knowledge: Health Sciences - Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Patricia Moreira de Freitas Costa e Silva
• Grantee: Camila Vieira da Silva
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Given that the protective capacity of the acquired pellicle in dental erosion can be influenced by several factors and the characteristic of this film can modify the deposition of salts on the surface of the enamel, it is possible that it can influence the effectiveness of the AmF/NaF/SnCl2 solution in inhibiting the progression of erosion. Thus, the in situ study was designed to evaluate if the acquired pellicle and timing of application of the AmF/NaF/SnCl2 solution interfere with its action. Twelve volunteers using a unilateral lower removable device containing 2 samples of human dental enamel will participate in this crossover in situ study, divided into four phases of 5 days each (split-mouth). Will be obtained from human third molars, 192 samples of human enamel (3 x 3 x 1 mm) which, after the formation of incipient erosive lesion (1% citric acid, pH 4.0, for 3 minutes), will be divided into 8 experimental groups (n = 12): G1 - Distilled water previously applied to the daily erosive cycling, without acquired pellicle; G2 - Distilled water applied at the end of erosive daily cycling, without acquired pellicle; G3 - G1 - Distilled water previously applied to the daily erosive cycling, with acquired pellicle; G4 - Distilled water applied at the end of erosive daily cycling, with acquired pellicle; G5 - AmF/NaF/SnCl2 solution previously applied to daily erosive cycling, without acquired pellicle; G6 - AmF/NaF/SnCl2 solution applied at the end of erosive daily cycling, without acquired pellicle; G7 - AmF/NaF/SnCl2 solution previously applied to daily erosive cycling, with acquired pellicle; AmF/NaF/SnCl2 solution applied at the end of erosive daily cycling, with acquired pellicle. During the experimental in situ phase the device containing the samples will be immersed in citric acid 0.5%, pH 2.6, 5 minutes, 6x/day at 1.5 hour intervals. At the end of each in situ experimental phase, the samples will be removed from the device and analyzed by optical profilometry test for determination of tissue loss and the average of duplicate samples will be considered for statistical analysis. The qualitative analysis of subsurface morphology will be seen through high resolution Scanning Electron Microscopy in combination with energy-dispersive X-ray, considering n = 3. The results obtained in profilometry will be evaluated according to the classical statistical concepts with 5% significance level. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, V, C.; MANTILLA, T. F.; ENGEL, Y.; TAVARES, J. P.; FREITAS, P. M.; RECHMANN, P.. The effect of CO2 9.3 mu m short-pulsed laser irradiation in enamel erosion reduction with and without fluoride applications-a randomized, controlled in vitro study. Lasers in Medical Science, v. 35, n. 5, FEB 2020. (15/10248-5, 16/08524-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Camila Vieira da. Influence of the acquired pellicle and the moment of application of the AmF/NaF/SnCl2 solution in the prevention of in situ erosion in human enamel. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.