Study of the effect of HDL on activated neutrophils

• Grant number: 17/17270-1
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2017
• End date: November 30, 2022
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Graziella Eliza Ronsein
• Grantee: Lorenna Rocha Reis
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 16/00696-3 - Proteomic as a tool to understand HDL function and composition, AP.JP

Abstract

In order to destroy invading pathogens, activated neutrophils generate reactive oxygen speciesand extracellular DNA networks associated with histones and myeloperoxidase (MPO), forming a trap to facilitate killing of the infectious agent. These networks were discovered in 2004, and called "NETs" (Neutrophil Extracellular Traps), with the neutrophil-triggered process named NETosis. The NETosis is a process of cell death distinct from apoptosis and necrosis. Recent data also indicates that NETosis can be characterized by either the conservation or rupture of the cytoplasmic membrane of the cell. Even more interesting is the fact that NETosis can happen on sterile conditions. For example, hyperlipidemia may induce neutrophil activation. In addition, activation of the neutrophils can be attenuated by HDL and APO A1, the main protein of HDL. Little is known about the mechanisms of sterile activation and release of NETs from neutrophils triggered by high concentrations of lipids. Also little is known about the effects of HDL and APO A1. In this way, we intend to evaluate which mediators of NETs in neutrophils are activated in sterile conditions (hyperlipidemia, for example), and if this process is similar to that triggered by infectious agents. Besides, we intend to investigate the modulating effect of HDL in the activation of these cells. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REIS, LORENNA ROCHA; JUNIOR, DOUGLAS RICARDO SOUZA; TOMASIN, REBEKA; BRUNI-CARDOSO, ALEXANDRE; DI MASCIO, PAOLO; RONSEIN, GRAZIELLA ELIZA. Citrullination of actin-ligand and nuclear structural proteins, cytoskeleton reorganization and protein redistribution across cellular fractions are early events in ionomycin-induced NETosis. REDOX BIOLOGY, v. 64, p. 14-pg., 2023-06-23. (13/07937-8, 16/00696-3, 17/17270-1, 19/25702-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

REIS, Lorenna Rocha. Investigation of the intrinsic mechanisms to NETs release through proteomics. 2023. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.