Comparative immunohistochemical characterization of subgroups of dendritic cells, macrophages and cytotoxic T lymphocytes in oral leukoplakia and proliferative verrucous leukoplakia

• Grant number: 17/17288-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2017
• End date: October 31, 2018
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Andreia Bufalino
• Grantee: Mariel Ruivo Biancardi
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Squamous cell carcinoma (SCC) accounts for more than 95% of all malignancies involving the oral cavity, and often these tumors are preceded by clinical alterations that have a clear potential for malignant transformation, which are denominated oral potentially malignant disorders (OPMDs). Among these, Oral Leukoplakia (OL) is the most important and has a malignant transformation rate ranging from 0.2% to 17.5%. Another important OPMD is Proliferative Verrucous Leukoplakia (PVL), which presents persistent and progressive malignant behavior, with a malignant transformation rate greater than 70%. Differently from OL, risk factors such as tobacco, alcohol and areca do not seem to be associated with the development of PVL. Additionally, a PVL presents an inadequate response to all treatment modalities, undergoes rapid spread for multiple oral sites and often presents recurrence. Recent studies and preliminary data obtained by our group suggest that the inflammatory infiltrate associated with the lesions of patients with PVL is related to the etiology and/or distinct clinical behavior from the conventional OL. Thus, this study intends to carry out a comparative analysis between OL and PVL samples consisting of: (1) evaluating a density and activation state of dendritic cells, (2) evaluating a density and polarization of macrophages M1 and M2, and (3) evaluate the percentage and identify the subtypes of T-helpers lymphocytes and activation state of T-cytotoxic lymphocytes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, DARCY; BARBEIRO, CAMILA DE OLIVEIRA; PALACON, MARIANA PARAVANI; BIANCARDI, MARIEL RUIVO; FERRISSE, TULIO MORANDIN; SILVEIRA, HEITOR ALBERGONI; CASTILHO, ROGERIO MORAES; DE ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE; BUFALINO, ANDREIA. High density of CD8 T cell and immune imbalance of T lymphocytes subsets are associated with proliferative verrucous leukoplakia. IMMUNOLOGY, v. 168, n. 1, p. 14-pg., 2022-09-03. (17/01798-7, 17/01438-0, 13/07276-1, 18/22236-0, 21/01191-0, 18/04954-2, 17/17288-8)

PALACON, MARIANA PARAVANI; BARBEIRO, CAMILA DE OLIVEIRA; FERNANDES, DARCY; BIANCARDI, MARIEL RUIVO; SILVEIRA, HEITOR ALBERGONI; FERRISSE, TULIO MORANDIN; LEON, JORGE ESQUICHE; KUJAN, OMAR; BUFALINO, ANDREIA. Macrophages CD163+and Factor XIIIa plus Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 6, p. 12-pg., 2023-03-01. (18/04954-2, 18/22236-0, 13/07276-1, 21/01191-0, 17/01798-7, 17/17288-8)