Comparative immunohistochemical analysis of dendritic cells, cytotoxic lymphocytes, and indolamine 2,3-dioxigenase expression in potentially malignant disorders and squamous cells carcinoma of the oral cavity and lower lip

• Grant number: 11/52090-8
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2012
• End date: June 30, 2014
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Jorge Esquiche León
• Grantee: Jorge Esquiche León
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The detection of epithelial dysplasia in oral leukoplakia (OL) and actinic cheilitis (AC) is the most important indicator for risk of squamous cell carcinoma (SCC), Unlike the lower lip SCC (LL-SCC), the oral cavity SCC (OSCC) is characterized by a high degree of local invasion and frequent lymph node metastasis. Despite great advances in the OSCC therapy, the 5-year survival rate has remained at less than 50% for the past 40 years. Several studies nave demonstrated that the immune system cannot only protect the host against tumor development, but also it has the ability to promote tumor growth, a dual effect of the immune system called "câncer imunoediting", in which the enzyme indoleamine 2,3 - dioxygenase (IDO) is directly involved. While these mechanisms in OL and AC are unknown, only two studies evaluating the expression of IDO in OSCC and LL-SCC have shown heterogeneous distribution. Such studies represent an excellent opportunity for a comparative analysis of the immune response in both potentially malignant disorders and SCC, besides to evaluate for effects of solar ultraviolet radiation. The purpose of this research project is to analyze, through an extensive immunohistochemical panel, the location and functional status of dendritic cells, cytotoxic lymphocytes and IDO expression in normal oral mucosa (n= 10), OL (n= 15), AC (n= 15), OSCC (n = 15) and LL-SCC (n = 10). All these cases will be retrieved from the files of the Histopathology Laboratory of the Faculty of Dentistry of Ribeirão Preto, University of São Paulo (FORP-USP). The results will help to better define the subtypes of cell activation and regulation of the immune system and its relationship with IDO expression, in order to contribute to the development of immunotherapeutic strategies for SCC treatment. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

YACARINI PAREDES, SILVIA ELENA; BEZERRA SEGATO, RAQUEL ASSED; MOREIRA, LEILA DAHER; MOREIRA, ALCIDES; DIAZ SERRANO, KRANYA VICTORIA; RODRIGUES, CLARISSA TELES; ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE. Dentoalveolar Abscesses Not Associated with Caries or Trauma: A Diagnostic Hallmark of Hypophosphatemic Rickets Initially Misdiagnosed as Hypochondroplasia. Head and Neck Pathology, v. 12, n. 4, p. 604-609, DEC 2018. (16/11419-0, 11/52090-8)

BEZERRA SILVA, DIEGO FILIPE; DE PONTES SANTOS, HELLEN BANDEIRA; LEON, JORGE ESQUICHE; DE CASTRO GOMES, DALIANA QUEIROGA; ALVES, POLLIANNA MUNIZ; WEEGE NONAKA, CASSIANO FRANCISCO. Análise clinicopatológica e imuno-histoquímica do carcinoma de células escamosas de células fusiformes da língua: um caso raro. Einstein (São Paulo), v. 17, n. 1, JAN-MAR 2019. (11/52090-8, 16/11419-0)

ORTEGA, ROSE MARA; BUFALINO, ANDREIA; ALMEIDA, LUCIANA YAMAMOTO; NAVARRO, CLAUDIA MARIA; TRAVASSOS, DAPHINE CAXIAS; FERRISSE, TULIO MORANDIN; CARLOS, ROMAN; LEON, JORGE ESQUICHE. Synchronous Polymorphous Adenocarcinoma and Canalicular Adenoma on the Upper Lip: An Unusual Presentation and Immunohistochemical Analysis. Head and Neck Pathology, v. 12, n. 1, p. 145-149, MAR 2018. (16/11419-0, 11/52090-8)

DOS SANTOS, JESSICA LUANA; DE ALMEIDA MILANI ALTEMANI, ALBINA MESSIAS; TRIVELLATO, ALEXANDRE ELIAS; SVERZUT, CASSIO EDVARD; ALMEIDA, LUCIANA YAMAMOTO; TEIXEIRA, LUCAS RIBEIRO; RIBEIRO-SILVA, ALFREDO; LEON, JORGE ESQUICHE. Intraoral Pigmented Low-Grade Adenocarcinoma, Not Otherwise Specified: Case Report and Immunohistochemical Study. Head and Neck Pathology, v. 12, n. 4, p. 610-618, DEC 2018. (16/11419-0, 11/52090-8)

ALMEIDA, LUCIANA YAMAMOTO; LOPES DOMINGUETE, MATHEUS HENRIQUE; DOMINGUETE, PAULO ROBERTO; RIBEIRO-SILVA, ALFREDO; TEIXEIRA, LUCAS RIBEIRO; LEON, JORGE ESQUICHE. Immune cell infiltration in Ectomesenchymal chondromyxoid tumor: An immunohistochemical study. Oral Oncology, v. 81, p. 112-115, JUN 2018. (16/11419-0, 11/52090-8)

DA MOTTA, RAPHAEL J. G.; TIRAPELLI, CAMILA; DA SILVA, ROBERTO JUNS; VILLAFUERTE, KELLY R. V.; ALMEIDA, LUCIANA Y.; RIBEIRO-SILVA, ALFREDO; LEON, JORGE E.. Immature, but Not Mature, Dendritic Cells Are More Often Present in Aggressive Periodontitis Than Chronic Periodontitis: An Immunohistochemical Study. Journal of Periodontology, v. 87, n. 12, p. 1499-1507, DEC 2016. (11/52090-8)

DOS SANTOS, JESSICA LUANA; MACEDO, RANDER MOREIRA; ALMEIDA, LUCIANA YAMAMOTO; SILVA, RODRIGO NEVES; RIBEIRO-SILVA, ALFREDO; LEON, JORGE ESQUICHE. Gingival melanoacanthoma associated with pseudomelanocytic nests: Expanding the clinicopathological spectrum of a recently described oral lesion. JOURNAL OF CUTANEOUS PATHOLOGY, v. 45, n. 9, p. 3-pg., 2018-09-01. (11/52090-8, 16/11419-0)