Rumen bacterial diversity and efficiency of nitrogen use in Nellore steers fed with different levels and sources of protein in the diet

• Grant number: 17/11571-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2017
• End date: February 28, 2019
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Telma Teresinha Berchielli
• Grantee: Kênia Larissa Gomes Carvalho Alves
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 16/22022-4 - Impact of metabolizable protein in the production of beef cattle and emission of greenhouse gases, AP.TEM

Abstract

The objective of this study was to characterize the ruminal bacterial community in Nellore steers fed with different levels and protein sources, and to identify possible associations between the ruminal microbiota profile and the variations in nitrogen (N) efficiency by the animal. Six Nellore steers distributed in a 6x6 Latin square will be used, with a 2x3 factorial arrangement (2 levels and 3 protein sources). Six experimental diets will be evaluated: diet urea (30% RDP substituted by urea) - 13% CP; diet urea (30% RDP) - 10% CP; diet with corn gluten 60 (82% RUP) - 13% CP; diet with corn gluten 60 - 10% CP; diet with dry beans by distillation (DDGS, 50% PNDR) - 13% CP; and dry grains diet by distillation (DDGS) - 10% CP. In all diets the source of bulky used will be cana in natura. The consumption, fecal and urinary excretion of N, as well as ruminal concentration of ammoniacal N and the efficiency of microbial N synthesis will be evaluated. Ruminal bacterial diversity will be assessed by sequencing the V4/V5 region of the bacterial 16S rRNA gene. The results will be analyzed statistically using Software R. The Tukey post-test will be used to compare the means, the nominal or binary nominal logistic regression test, as appropriate, will be used to determine the possible effect of the abundance of the identified ruminal bacterial phyla, families or genus on excretion and retention of nitrogen by the animal and the relationship between these results will be determined by the Pearson correlation. In all tests the significance will be declared at P d 0.05. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES CARVALHO ALVES, KENIA LARISSA; GRANJA-SALCEDO, YURY TATIANA; MESSANA, JULIANA DUARTE; DE SOUZA, VINICIUS CARNEIRO; GENEROSO GANGA, MARIA JULIA; DETOGNI COLOVATE, PAULO HENRIQUE; KISHI, LUCIANO TAKESHI; BERCHIELLI, TELMA TERESINHA. Rumen bacterial diversity in relation to nitrogen retention in beef cattle. Anaerobe, v. 67, FEB 2021. (17/02034-0, 16/16796-7, 18/13885-4, 16/22022-4, 17/11571-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALVES, Kênia Larissa Gomes Carvalho. Rumen bacterial diversity and efficiency of nitrogen use in Nellore steers fed with different levels and sources of protein in the diet. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.