The effects of the Alamandine on the Na+/H+ exchanger in in vivo proximal tubule of spontaneously hypertensive rats

• Grant number: 17/10772-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): December 01, 2017
• Effective date (End): April 30, 2022
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Gerhard Malnic
• Grantee: Regiane Cardoso Castelo Branco
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 18/25438-2 - Role of alamandineE/MrgD interaction in acute kidney injury, BE.EP.PD

Abstract

The Renin Angiotensin System (RAS) is a regulator of the cardiovascular system and the electrolyte balance. A new component of the system has recently been identified, an alamandine, a heptapeptide generated by the catalytic action of the angiotensin-converting enzyme 2 or directly from Angiotensin-(1-7) [Ang-(1-7)]. Alamandine produces several physiological actions similar to those produced by Ang-(1-7), including vasodilation, antifibrosis, antihypertensive, and central effects. So far, Alamandine's actions on the kidney are not well understood. In addition, the important role of RAS in the pathophysiology of cardiovascular and renal diseases is widely recognized, and it is recognized that in these diseases the beneficial effects of Alamandine are similar to the protective effects of Ang-(1-7). To contribute to the understanding of the etiology of arterial hypertension, we intend to investigate the effects of Alamandine and/or the antagonist of the AT2 receptor of Angiotensin II PD123319 in tubular microperfusion in spontaneously hypertensive rats (SHR). The physiology of the organ will be evaluated by the quantification of the isoform 3 expression of the Na+/H+ exchanger (NHE3, responsible for the regulation of the intra- and extracellular volume) and the AT2 and MAS receptors by Western blotting of the renal tissue.