Investigation of spleen participation in the cholinergic anti-inflammatory pathway during the wound healing process in mice

• Grant number: 17/12025-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2017
• End date: November 30, 2018
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Hosana Gomes Rodrigues
• Grantee: Renato da Silva Cardoso
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 13/06810-4 - Mechanisms of action of omega-3 and omega-6 in the tissue repair process: neuro-immune focus, AP.JP

Abstract

The skin, the largest organ of the human body, is considered the main and first barrier of protection of the organism against external agents and, therefore, is subject to constant aggressions, making its repairing capacity very important, not only for the maintenance of local homeostasis, but also for the survival of the organism. Wound healing is a physiological process divided into 3 overlapping and concomitant phases: inflammation, proliferation and maturation. Considering that the inflammatory response comprises the first phase of this process, mechanisms of inflammatory control may affect the healing process. In recent years it has been described that the central nervous system, through the vagus nerve, can control the inflammatory response. However, it is not yet clear how this control occurs in tissues not innervated by the vagus nerve, such as the skin. One of the hypotheses is that this would occur through the participation of the spleen. In this sense, based on results already described in the literature, which suggest the possible essentiality of the spleen in the cholinergic anti-inflammatory pathway during the neuromodulation of inflammatory processes; In the present study, we sought to determine the participation of the spleen in the cholinergic anti-inflammatory pathway during the wound healing process in mice. To do this, we will induce wounds in the dorsal region of male C57black / 6 mice and they will be divided into 6 groups: (0) 0 hours; (1d) 1day; (3d) 3 days; (7d) 7 days; (10d) 10 days and (21d) 21 days, which correspond to the times after the wounds in which the animals will be euthanized and their spleens will be collected. After these procedures, the spleens will be homogenized and cytokine production will be evaluated by ELISA, as well as, cell phenotyping and expression of certain cellular receptors in the spleen will be evaluated by flow cytometry. In addition to these, C57black / 6 male mice will be divided into 2 other groups: (C) control animals that will receive intraperitoneal (ip) injection of PBS or PNU 282987, a specific nicotinic ±7 -receptor agonist (receptor responsible for pathway activation), which will have their spleens collected after 1 and 3 days for the quantification of cytokines, also, by the ELISA methodology. The data will be analyzed by ANOVA and Tukey post-test. (AU)