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Ecotoxicological aspects of Tartrazine dye on the Danio rerio species before and after the use of different classes of treatments

Grant number: 17/08106-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2017
Effective date (End): October 31, 2018
Field of knowledge:Biological Sciences - Ecology - Applied Ecology
Principal Investigator:Renata Fracácio Francisco
Grantee:Samara Cristina Freire da Silva
Host Institution: Instituto de Ciência e Tecnologia. Universidade Estadual Paulista (UNESP). Campus de Sorocaba. Sorocaba , SP, Brazil


Pollution of aquatic environments by inorganic and organic chemicals is one of the main factors that is shown to be a serious threat to the survival of the biota present in said system. Due to the growth of industrial production in the last decades the manufacture and use of synthetic chemical compounds has been intensively increased. In this context, we highlight the textile and food industries that generate complex effluents, containing among other chemical compounds, the dyes, designed to not degrade easily. Currently more than 4,000 dyes have been tested, and more than 90% of them presented high toxicity values, highlighting a group of dyes called azo dyes, characterized by the connections between nitrogen giving these molecules a great stability and low natural degradation. Among the azo dyes widely used in industrial sectors that require color, we can highlight the Tartrazine dye, an artificial synthetic organic yellow dye whose permissible concentration does not compromise the multiple uses of water, considering the classification of water bodies (CONAMA 357/05) and emphasizing the protection of aquatic life, does not appear in current Brazilian legislation. In this context it is desirable that the active principles contained in industrial effluents do not have the potential to cause toxicity to aquatic biota (CONAMA 430/11). In view of the above, the present research aims to evaluate the chemical degradation of the dye by means of spectrophotometry and the reduction of the acute toxicity of the same in aqueous solution, before and after the following treatments: 1) Ultrasound; 2) Coal from sugarcane powder; 3) Advanced Oxidative Process with titanium dioxide (TiO2) and fluorescent light (POA) and 4) Bioremediation by fungi of the species Pleorotus ostreatus. For the study of reduction of acute toxicity, adult fish of the species Danio rerio (2 to 3 months of age) will be used, following the ABNT NBR 15088 (2011) standard. Through the statistical analysis of Kruskal-Wallis the efficiency of treatments for the removal of acute toxicity will be analyzed. (AU)

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