Development of electrochemical sensor to monitoring hydrogen peroxide in cellular media during processes of induced oxidative stress

• Grant number: 17/21585-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2018
• End date: December 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Mauro Bertotti
• Grantee: Eduardo José de Carvalho Junior
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This work aims at the monitoring of the concentration of excreted hydrogen peroxide by keratinocyte cells, HaCaT line, during oxidative stress processes induced by 1,9- dimethyl methylene blue, DMMB, by using radiation with wavelength of 631 nm. The amperometric monitoring of hydrogen peroxide in cell culture media will be performed with a microsensor developed specifically for this purpose, which will consist of a gold microelectrode whose surface will be activated by the generation of a nanoporous structure. Due to de complexity of the matrix, denominated "Dulbecoo's Modified Eagle Medium", DMEM, preliminary studies will be carried out with the aim of evaluating the selectivity of analytical determinations of hydrogen peroxide. The experiments will be conducted at room temperature and at 37°C. The work will be carried out in collaboration with the research team of Prof. Dr. Mauricio S. Baptista, from the Department of Biochemistry, which will be responsible for the maintenance and incubation of the cell cultures with DMMB and the experiments involving irradiation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SUKERI, ANANDHAKUMAR; DE CARVALHO JUNIOR, EDUARDO JOSE; BERTOTTI, MAURO. A novel approach for one-step fabrication of platinum-nanoporous gold film via oxygen bubble template with enhanced electrochemical activity. Electrochemistry Communications, v. 100, p. 96-99, MAR 2019. (18/08782-1, 17/21585-8)