Development of a fluorescence-based approach for the high-throughput isolation of Kluyveromyces marxianus recombinant haploids

• Grant number: 17/24957-3
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: April 16, 2018
• End date: July 15, 2018
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Gonçalo Amarante Guimarães Pereira
• Grantee: Monique Furlan
• Supervisor: Jamie Cate
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: University of California, Berkeley (UC Berkeley), United States
• Associated to the scholarship: 16/12852-0 - Implementation of a tetrad dissection method for Saccharomyces cerevisiae based on flow cytometry, BP.IC

Abstract

Yeasts adapted to industrial conditions are considered appropriate platforms for the development of commercial strains applied to the production of chemicals derived from renewable raw materials, in particular biofuels. In this context, laboratory evolution based on sexual hybridization is considered a powerful complementary approach to metabolic engineering. However, understanding genetic mechanisms related to robustness or combining industrially relevant characteristics by crosses requires the collection and analysis of a large number of segregants. In Kluyveromyces marxianus, obtaining haploids is typically done manually by tetrad dissection, an extremely laborious procedure depending on the number of haploids needed. In this context, the implementation of high-throughput tetrad dissection techniques has a major impact in reducing the time required to study or develop new strains. For this reason, we intend to implement a method of analysis and isolation of tetrads based on flow cytometry and apply it in the study of genes related to thermotolerance, a feature widely observed in this yeast specie. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CERNAK, PAUL; ESTRELA, RAISSA; PODDAR, SNIGDHA; SKERKER, JEFFREY M.; CHENG, YA-FANG; CARLSON, ANNIKA K.; CHEN, BERLING; GLYNN, VICTORIA M.; FURLAN, MONIQUE; RYAN, OWEN W.; et al. Engineering Kluyveromyces marxianus as a Robust Synthetic Biology Platform Host. MBIO, v. 9, n. 5, SEP-OCT 2018. (17/24957-3)