Immediate and long-term evaluation of the adhesive interface after dentin biomodification with chitosan solution and application of different adhesive systems

• Grant number: 17/21855-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2017
• End date: November 30, 2018
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Aline Evangelista de Souza Gabriel
• Grantee: Vitória Leite Paschoini
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Biomodification of dentin collagen with biopolymers have been proposed as a strategy to preserve the hybrid layer. The aim of this study is to evaluate, in vitro, the bond strength of the adhesive interface and to characterize it chemically and morphologically, after the treatment of dentin with chitosan solution and application of different adhesive systems, in immediate and long-term periods. The sample will be composed of 80 healthy third molars, which will be divided into 2 groups according to the dentin treatment: without biomodification (control) and biomodified with 2.5% chitosan solution, and subdivided into 2 according to the adhesive systems: total-etching (Adper Single Bond 2, 3M) and self-etching (ClearFil SE Bond, Kuraray). The dental surface will be restored with composite resin (Filtek Z250, 3M) and half of the teeth of each subgroup will be subjected to the interface aging (6 months of storage in water + enzymatic degradation) and the other half will be used for immediate analysis (24h) (n=10). The teeth will be sectioned into sticks (transversal area of 1.0 ± 0.2 mm²) and subjected to the following analyzes: 1) microtensile bond strength (MPa); 2) degree of conversion of the adhesive system (%) by means of FTIR; 3) chemical composition (%) of the adhesive interface by EDS and 4) morphology of the adhesive interface by SEM. The selection of statistical tests will be based on data set characteristics such as adherence to normal curve and homocedasticity. The statistical inferences will be done considering the significance level of 5%.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

PASCHOINI, VITORIA LEITE; ZIOTTI, ISABELLA RODRIGUES; NERI, CLAUDIO ROBERTO; MILORI CORONA, SILMARA APARECIDA; SOUZA-GABRIEL, ALINE EVANGELISTA. Chitosan improves the durability of resin-dentin interface with etch-and-rinse or self-etch adhesive systems. Journal of Applied Oral Science, v. 29, 2021. (17/21855-5)