| Grant number: | 17/23254-9 |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| Start date: | March 01, 2018 |
| End date: | February 28, 2019 |
| Field of knowledge: | Physical Sciences and Mathematics - Chemistry - Analytical Chemistry |
| Principal Investigator: | Joaquim de Araújo Nóbrega |
| Grantee: | Legna Andreina Colina Vegas |
| Supervisor: | Frank Vanhaecke |
| Host Institution: | Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil |
| Institution abroad: | Ghent University (UGent), Belgium |
| Associated to the scholarship: | 16/23130-5 - Determination of cellular biodistribution of Cu, Ru and Pt in human tumor and non tumor breast cells by Inductively Coupled Plasma Mass Spectrometry, BP.PD |
Abstract For years, cancer has been the second cause of death worldwide, only being preceded by cardiovascular diseases. An increasing amount of research groups is focusing on the discovery of new and more effective anti-cancer drugs that induce less severe side effects and encounter less therapeutic resistance. In this context, compounds containing transition metals, in particular complexes containing copper, ruthenium and platinum, have been proposed as antitumor agents that present anti-proliferative and anti-metastatic behavior. Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is a powerful analytical technique to perform either quantitative bulk elemental analysis or spatially resolved elemental imaging/mapping of elements in biological tissues, including populations of single cells. Preliminary studies have demonstrated the high cytotoxic potential of the complexes [Cu(CTZ)2(PPh3)2]PF6, [Ru(p-cym)(CTZ)(PPh3)Cl]PF6 and cis-[Pt(CTZ)(PPh3)2Cl]PF6, (where CTZ: clotrimazole, p-cym: p-cymene and PPh3: triphenylphosphine) against the human breast carcinoma cell line MDA-MB-231. This project aims to determine mean concentrations of Cu, Ru and Pt in tumor cell populations, exposed to different concentrations of the compounds, and to reveal the two-dimensional compound distribution in single cells by LA-ICP-MS analysis. Furthermore, it is expected that by means of combining chemical and biological data, it would be possible to elucidate their mechanism of action as well as a correlation between the structure and activity of the compounds tested. | |
| News published in Agência FAPESP Newsletter about the scholarship: | |
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