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Quantitative determination and cellular imaging of Cu, Ru and Pt in single cells using Laser Ablation-ICP-Mass Spectrometry

Grant number: 17/23254-9
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): March 01, 2018
Effective date (End): February 28, 2019
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Joaquim de Araújo Nóbrega
Grantee:Legna Andreina Colina Vegas
Supervisor abroad: Frank Vanhaecke
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Research place: Ghent University (UGent), Belgium  
Associated to the scholarship:16/23130-5 - Determination of cellular biodistribution of Cu, Ru and Pt in human tumor and non tumor breast cells by Inductively Coupled Plasma Mass Spectrometry, BP.PD


For years, cancer has been the second cause of death worldwide, only being preceded by cardiovascular diseases. An increasing amount of research groups is focusing on the discovery of new and more effective anti-cancer drugs that induce less severe side effects and encounter less therapeutic resistance. In this context, compounds containing transition metals, in particular complexes containing copper, ruthenium and platinum, have been proposed as antitumor agents that present anti-proliferative and anti-metastatic behavior. Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is a powerful analytical technique to perform either quantitative bulk elemental analysis or spatially resolved elemental imaging/mapping of elements in biological tissues, including populations of single cells. Preliminary studies have demonstrated the high cytotoxic potential of the complexes [Cu(CTZ)2(PPh3)2]PF6, [Ru(p-cym)(CTZ)(PPh3)Cl]PF6 and cis-[Pt(CTZ)(PPh3)2Cl]PF6, (where CTZ: clotrimazole, p-cym: p-cymene and PPh3: triphenylphosphine) against the human breast carcinoma cell line MDA-MB-231. This project aims to determine mean concentrations of Cu, Ru and Pt in tumor cell populations, exposed to different concentrations of the compounds, and to reveal the two-dimensional compound distribution in single cells by LA-ICP-MS analysis. Furthermore, it is expected that by means of combining chemical and biological data, it would be possible to elucidate their mechanism of action as well as a correlation between the structure and activity of the compounds tested.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VAN ACKER, THIBAUT; VAN MALDEREN, STIJN J. M.; COLINA-VEGAS, LEGNA; RAMACHANDRAN, RANJITH K.; VANHAECKE, FRANK. Selective ablation of biological tissue and single cells on a glass substrate by controlling the laser energy density of nanosecond 193 nm laser radiation. Journal of Analytical Atomic Spectrometry, v. 34, n. 10, p. 1957-1964, OCT 1 2019. Web of Science Citations: 0.
COSTAS-RODRIGUEZ, MARTA; COLINA-VEGAS, LEGNA; SOLOVYEV, NIKOLAY; DE WEVER, OLIVIER; VANHAECKE, FRANK. Cellular and sub-cellular Cu isotope fractionation in the human neuroblastoma SH-SY5Y cell line: proliferating versus neuron-like cells. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 411, n. 19, SI, p. 4963-4971, JUL 2019. Web of Science Citations: 2.
COLINA-VEGAS, LEGNA; PRADO GODINHO, JOSEANE LIMA; COUTINHO, THALLITA; CORREA, RODRIGO S.; DE SOUZA, WANDERLEY; FERNANDES RODRIGUES, JULIANY COLA; BATISTA, ALZIR AZEVEDO; NAVARRO, MARIBEL. Antiparasitic activity and ultrastructural alterations provoked by organoruthenium complexes against Leishmania amazonensis. NEW JOURNAL OF CHEMISTRY, v. 43, n. 3, p. 1431-1439, JAN 21 2019. Web of Science Citations: 1.
COLINA-VEGAS, LEGNA; OLIVEIRA, KATIA M.; CUNHA, BEATRIZ N.; COMINETTI, MARCIA REGINA; NAVARRO, MARIBEL; BATISTA, ALZIR AZEVEDO. Anti-Proliferative and Anti-Migration Activity of Arene-Ruthenium(II) Complexes with Azole Therapeutic Agents. INORGANICS, v. 6, n. 4 DEC 2018. Web of Science Citations: 2.

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