Exploring the Biophysical Properties of Human Golgi Reassembly and Stacking Protein 55 in Solution and Its Interaction with Model Membranes

• Grant number: 17/12146-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2018
• End date: March 31, 2021
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Antonio José da Costa Filho
• Grantee: Thirupathi Reddy Soudherpally
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Golgi Reassembly and Stacking Proteins (GRASPs) play a significant role in a variety of functions including the formation of ribbon-like Golgi apparatus and secretion of unconventional proteins. The molecular mechanism underlying the functional properties of GRASPs remains unaddressed. Our project mainly focuses on exploring the biochemical and biophysical characterization of the human full-length GRASP 55 in solution and its interaction with model membranes. Although various crystal structures of GRASPs have been reported, there is a lack of data in understanding the structure-activity relationship. In the present project, we aim to address two major aspects of the mechanistic basis of GRASP 55 function. (1) Although GRASPs (such as GRASP 55) are involved in various functions, the comprehensive understanding of structure, size, oligomerization, and intrinsically disordered protein (IDP) behavior are not known. (2) GRASPs are involved in tethering of Golgi membranes, however, there are no reports so far on the interaction of GRASPs with Golgi membranes. To carry out these studies, we propose to use a combination of biochemical and biophysical methods such as proteolysis assays, circular dichroism and fluorescence spectroscopy, small angle X-ray scattering, differential scanning calorimetry, solution nuclear magnetic resonance, electron spin resonance, and isothermal titration calorimetry. We envision that insights from our experiments would help in extending our present understanding of the structural aspects of GRASP 55 and its interaction with model membranes which are crucial for its function.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENDES, LUIS F. S.; FONTANA, NATALIA A.; REDDY, S. THIRUPATHI; UVERSKY, VLADIMIR N.; COSTA-FILHO, ANTONIO J.. The exquisite structural biophysics of the Golgi Reassembly and Stacking Proteins. International Journal of Biological Macromolecules, v. 164, p. 3632-3644, DEC 1 2020. (12/20367-3, 15/50366-7, 16/23863-2, 17/24669-8, 17/12146-0)

REDDY, S. THIRUPATHI; UVERSKY, VLADIMIR N.; COSTA-FILHO, ANTONIO JOSE. Nucleation-dependent amyloid fibrillation of human GRASP55 in aqueous solution. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, v. 49, n. 2, p. 133-143, MAR 2020. (12/20367-3, 17/12146-0, 15/50366-7)

THIRUPATHI REDDY, S.; SANTOS MENDES, LUIS FELIPE; FONTANA, NATALIA APARECIDA; COSTA-FILHO, ANTONIO JOSE. Exploring structural aspects of the human Golgi matrix protein GRASP55 in solution. International Journal of Biological Macromolecules, v. 135, p. 481-489, AUG 15 2019. (16/23863-2, 17/12146-0, 17/24669-8, 15/16812-0, 15/50366-7, 12/20367-3)

REDDY, S. THIRUPATHI; UVERSKY, VLADIMIR N.; COSTA-FILHO, ANTONIO J.. Biophysical characterization of intrinsically disordered human Golgi matrix protein GRASP65. International Journal of Biological Macromolecules, v. 162, p. 1982-1993, NOV 1 2020. (15/50366-7, 17/12146-0, 15/16812-0, 12/20367-3)