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Electron magnetic resonance in molecular biophysics: new and old looks to new and old problems

Grant number: 15/18390-5
Support type:Regular Research Grants
Duration: April 01, 2016 - March 31, 2018
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal Investigator:Antonio José da Costa Filho
Grantee:Antonio José da Costa Filho
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The understanding the triad structure-dynamics-function leads to the most detailed description of processes involving biological molecules, in particular proteins. In this research proposal, we aim at using a combination of more traditional with more modern methods of Electron Magnetic Resonance (EMR), towards the determination of distance constraints and the detection of coexistence of molecular conformations, to investigate more traditional (such as peptide/membrane interactions) as well as more recent (such as intrinsically disordered proteins) problems in Molecular Biophysics. To do so, this proposal is divided in two problems: (I) the molecular interactions taking place in the functional mechanism of proteins and (II) fusion peptides from the glycoprotein S of SARS-CoV and their interactions with model membranes. In Problem I, we are interested in the human calcium binding protein S100A12 and in the Golgi Reassembly and Stacking Proteins (GRASPs). The first one is a more traditional problem (od problem) of searching for protein conformational changes in the presence of ligands (in this case, divalent ions), but exploited by using simulations of the EMR spectra and distance measurements (old and new looks). The second case is a new problem involving GRASP proteins that have shown features related to structural intrinsic disorder, a theme of research that has been expanding vigorously over the last years. GRASPs will be investigated by using the traditional look of CW-EMR combined with several other biophysical tools, and with potential for distance measurements through pulsed EMR. In Problem II, we are interested in the interactions between model membranes and low molecular mass ligands, such as biologically active peptides, drugs and metal complexes with antitumor activity. In this case, EMR will be jointly used, when necessary, with other methods, such as calorimetry, circular dichroism (CD) and fluorescence. This part of the project encompasses more traditional looks (spectroscopies in general) to more traditional problems, but with the addition of one new look, which is the use of pulsed EMR for distance measurements. The expected contributions are related not only to the specific issues of the problems, but also to the consolidation, in our state, of modern EMR methods, such as site directed spin labeling and distance measurements using double electron-electron resonance. (AU)

Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARROSO, RAFAEL P.; BERLIM, LEONARDO S.; ITO, AMANDO S.; COSTA-FILHO, ANTONIO J. In vitro antioxidant properties of golden grass (Syngonanthus nitens) by electron paramagnetic resonance. FOOD SCIENCE & NUTRITION, v. 7, n. 4, p. 1353-1360, APR 2019. Web of Science Citations: 0.
FONTANA, N. A.; FONSECA-MALDONADO, R.; MENDES, L. F. S.; MELEIRO, L. P.; COSTA-FILHO, A. J. The yeast GRASP Grh1 displays a high polypeptide backbone mobility along with an amyloidogenic behavior. SCIENTIFIC REPORTS, v. 8, OCT 24 2018. Web of Science Citations: 3.
MENDES, LUIS F. S.; BASSO, LUIS G. M.; KUMAGAI, PATRICIA S.; FONSECA-MALDONADO, RAQUEL; COSTA-FILHO, ANTONIO J. Disorder-to-order transitions in the molten globule-like Golgi Reassembly and Stacking Protein. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1862, n. 4, p. 855-865, APR 2018. Web of Science Citations: 4.
VICENTE, EDUARDO F.; SAHU, INDRA D.; CRUSCA, JR., EDSON; BASSO, LUIS G. M.; MUNTE, CLAUDIA E.; COSTA-FILHO, ANTONIO J.; LORIGAN, GARY A.; CILLI, EDUARDO M. HsDHODH Microdomain-Membrane Interactions Influenced by the Lipid Composition. Journal of Physical Chemistry B, v. 121, n. 49, p. 11085-11095, DEC 14 2017. Web of Science Citations: 1.
FONSECA-MALDONADO, RAQUEL; MELEIRO, LUANA P.; MENDES, LUIS F. S.; ALVES, LUANA F.; CARLI, SIBELI; MORERO, LUCAS D.; BASSO, LUIS G. M.; COSTA-FILHO, ANTONIO J.; WARD, RICHARD J. Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced beta-glucanase activity monitored by electron paramagnetic resonance. BIOTECHNOLOGY FOR BIOFUELS, v. 10, NOV 14 2017. Web of Science Citations: 4.
DE PADUA, RICARDO A. P.; KIA, ALI MARTIN; COSTA-FILHO, ANTONIO J.; WILKINSON, SHANE R.; NONATO, M. CRISTINA. Characterisation of the fumarate hydratase repertoire in Trypanosoma cruzi. International Journal of Biological Macromolecules, v. 102, p. 42-51, SEP 2017. Web of Science Citations: 4.
MICHELETTO, MARIANA C.; MENDES, LUIS F. S.; BASSO, LUIS G. M.; FONSECA-MALDONADO, RAQUEL G.; COSTA-FILHO, ANTONIO J. Lipid membranes and acyl-CoA esters promote opposing effects on acyl-CoA binding protein structure and stability. International Journal of Biological Macromolecules, v. 102, p. 284-293, SEP 2017. Web of Science Citations: 2.
VIEIRA, ERNANNI D.; BASSO, LUIS G. M.; COSTA-FILHO, ANTONIO J. Non-linear van't Hoff behavior in pulmonary surfactant model membranes. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1859, n. 6, p. 1133-1143, JUN 2017. Web of Science Citations: 3.
BASSO, LUIS G. M.; VICENTE, EDUARDO F.; CRUSCA, JR., EDSON; CILLI, EDUARDO M.; COSTA-FILHO, ANTONIO J. SARS-CoV fusion peptides induce membrane surface ordering and curvature. SCIENTIFIC REPORTS, v. 6, NOV 28 2016. Web of Science Citations: 9.

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