MicroRNAs and oxidized biomolecules as possible biomarkers of primary antiphospholipid syndrome

• Grant number: 17/19251-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2018
• End date: February 29, 2020
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Patricia Moriel
• Grantee: Camila de Oliveira Vaz
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 16/14172-6 - Investigation of the pathophysiological aspects and novel therapeutic approaches for thromboembolic disorders, AP.TEM

Abstract

An Antiphospholipid Antibody syndrome (APS) is an autoimmune disease which has as main characteristic the recurrence of idiopathic thrombotic events associated with the presence of anti-phospholipid antibodies (aPL). It is estimated that affects 2-4% of the world population; Being thus associated with 20% of cases of deep venous thrombosis, gestational loss and stroke ischemic stroke (CVA) in people under 45 years of age. However, mechanisms and pathophysiology has not yet been fully elucidated. Some recent study suggest that oxidative stress plays a fundamental role in the progression of the disease, mainly due to the depletion of antioxidant defenses and adjuvant in the establishment of a pro-inflammatory environment, culminates in endothelial dysfunction and did of platelet aggregation. It is further believed that the available thromboembolic process is epigenetically controlled, by the expression of specific miRNAs capable of interfere in the pro-coagulant environment. This work has the objective of identify miRNAs and oxidized biomolecules as biomarkers of SAF, associating those with a progression of disease, adherence to treatment and quality of life of patient’s use of anticoagulant therapy accompanied by ambulatory Hemocentro/UNICAMP. For this, plasma samples were collected from 80 patients with diagnosis of primary APS, matched by sex and age with healthy controls, for the quantification of oxidized biomolecules and extraction of miRNAs. After this quantification, such-miRNAs will be analyzed by microarrays, validated and evaluated by bioinformatics. Quality of life and adherence to anticoagulant therapy will be evaluated by applying the valid Portuguese version of the SF-36, DASS, MAT and MedTake. All results and correlations are statistically with p à 0.05 (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VAZ, CAMILA DE O.; MAZETTO, BRUNA M.; VASCONCELOS, PEDRO EDUARDO NASCIMENTO SILVA; BASTOS, LARISSA BRITO; CURSINO, MARIA APARECIDA; QUINTANILHA, JULIA COELHO FRANCA; MESQUITA, GABRIELA LISIANE TRIPIQUIA VECHIATTO; DOS SANTOS, ANA PAULA ROSA; JACINTHO, BRUNA CARDOSO; OLIVEIRA, JOSE DIOGO; et al. Answer to "REPLY to Association between Plasmatic Oxidative Stress and Thrombosis in Primary Antiphospholipid Syndrome". JOURNAL OF THROMBOSIS AND THROMBOLYSIS, v. 54, n. 1, p. 2-pg., 2022-04-13. (16/14172-6, 17/19251-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VAZ, Camila de Oliveira. Evaluation of oxidized biomolecules and microRNAs association with primary antiphospholipid syndrome thrombosis-related. 2020. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.