Development of fiber optic sensor for reflectance and light scattering monitoring for microfluidics applied to biotechnology

• Grant number: 17/20445-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2018
• End date: February 29, 2020
• Field of knowledge: Engineering - Electrical Engineering - Electrical, Magnetic and Electronic Measurements, Instrumentation
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Eric Fujiwara
• Grantee: Marco César Prado Soares
• Host Institution: Faculdade de Engenharia Mecânica (FEM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Microfluidics is the science which studies the manipulation of small fluid samples in micrometric channels, and has the purpose of remodeling conventional procedures, using systems with more compact and versatile shapes. It is a knowledge field that is still in the beginning of its development, but that is already capable of offering a series of advantages in relation to traditional macroscopic processes. Microfluidics applied to biotechnology has been receiving increasing attention and, for the experiments achieve the best results, it is necessary to provide an efficient analysis method. Today, few monitoring and sensing techniques are applicable, and the use of microscopy with fluorescence markers is the main technique to evaluate cell growth in biological systems. Due to some advantages intrinsic to fiber optics, such as robustness, low cost and ease of operation, some studies on the use of fiber optic sensors applied to microfluidic systems have already begun. Based on preliminary results, which indicated the phenomenon of dynamic light scattering in Saccharomyces cerevisiae cultures operating in perfusion mode, this project seeks to apply fiber optic sensing to a microfluidic device, providing a simpler alternative to the professionals who deal with these equipments daily. For this, data of microbial kinetics will be obtained through the analysis of optical microscopy, and these data, in turn, will be compared to the parameters collected with the fiber optic sensor. In order to perform the experiments, the flow rates and the temperature of the device will be kept constant and, starting from a given initial concentration of cells, the response will be verified when the microorganisms are submitted to different concentrations of limiting substrate. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PRADO SOARES, MARCO CESAR; MENDES, BEATRIZ FERREIRA; SCHENKEL, EGONT ALEXANDRE; SANTOS, MURILO FERREIRA; FUJIWARA, ERIC; SUZUKI, CARLOS KENICHI. Kinetic and Thermodynamic Study in Pozzolanic Chemical Systems as an Alternative for Chapelle Test. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 21, n. 4, 2018. (15/02185-3, 17/20445-8)

MARCO CÉSAR PRADO SOARES; MATHEUS KAUÊ GOMES; EGONT ALEXANDRE SCHENKEL; MATHEUS DOS SANTOS RODRIGUES; CARLOS KENICHI SUZUKI; LUCIMARA GAZIOLA DE LA TORRE; ERIC FUJIWARA. EVALUATION OF SILICA NANOPARTICLE COLLOIDAL STABILITY WITH A FIBER OPTIC QUASI-ELASTIC LIGHT SCATTERING SENSOR. Brazilian Journal of Chemical Engineering, v. 36, n. 4, p. 1519-1534, 2020-01-13. (17/06190-7, 17/20445-8)

VIT, FRANCIELE FLORES; NUNES, RONY; WU, YU TZU; SOARES, MARCO CESAR PRADO; GODOI, NAIARA; FUJIWARA, ERIC; CARVALHO, HERNANDES F.; DE LA TORRE, LUCIMARA GAZIOLA. A modular, reversible sealing, and reusable microfluidic device for drug screening. Analytica Chimica Acta, v. 1185, NOV 15 2021. (17/20445-8)

SOARES, MARCO C. P.; PERLI, GABRIEL; BARTOLI, JULIO R.; BERTUZZI, DIEGO L.; TAKETA, THIAGO B.; BATAGLIOLI, ROGDRIO A.; SUZUKI, CARLOS K.; ORNELAS, CATIA; FUJIWARA, ERIC; IEEE. Fast Microwave-Assisted Synthesis of Green-Fluorescent Carbon Nanodots from Sugarcane Syrup. 2019 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC), v. N/A, p. 5-pg., 2019-01-01. (17/20445-8)

SOARES, MARCO C. P.; VIT, FRANCIELE F.; DE LA TORRE, LUCIMARA G.; FUJIWARA, ERIC; IEEE. Online Monitoring of Cell Growth on PDMS-PDMS Reversible Microfluidic Bioreactor Integrated to Optical Fiber Sensor. 2019 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC), v. N/A, p. 5-pg., 2019-01-01. (17/20445-8)

SOARES, MARCO C. P.; MENDES, BEATRIZ F.; GOMES, MATHEUS K.; BATAGLIOLI, ROGERIO A.; SCHENKEL, EGONT A.; VIT, FRANCIELE F.; DE LA TORRE, LUCIMARA G.; FUJIWARA, ERIC; SUZUKI, CARLOS K.; IEEE. Optical Fiber Sensor as an Alternative for Colorimetric Image Processing for the Assessment of Dye Concentration. 2018 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC), v. N/A, p. 5-pg., 2018-01-01. (17/06190-7, 17/20445-8)

FUJIWARA, ERIC; GOMES, MATHEUS K.; SOARES, MARCO C. P.; RODRIGUES, MATHEUS S.; SCHENKEL, EGONT A.; SUZUKI, CARLOS K.; IEEE. Characterization of Colloidal Silica by Optical Fiber Sensor. 2018 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC), v. N/A, p. 5-pg., 2018-01-01. (17/06190-7, 17/20445-8)

PRADO SOARES, MARCO CESAR; VIT, FRANCIELE FLORES; SUZUKI, CARLOS KENICHI; DE LA TORRE, LUCIMARA GAZIOLA; FUJIWARA, ERIC. Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters. SENSORS, v. 19, n. 11, JUN 1 2019. (17/20445-8)

PRADO SOARES, MARCO CESAR; RODRIGUES, MATHEUS SANTOS; SCHENKEL, EGONT ALEXANDRE; PERLI, GABRIEL; ARITA SILVA, WILLIAN HIDEAK; GOMES, MATHEUS KAUE; FUJIWARA, ERIC; SUZUKI, CARLOS KENICHI. Evaluation of Silica Nanofluids in Static and Dynamic Conditions by an Optical Fiber Sensor. SENSORS, v. 20, n. 3, FEB 2020. (17/20445-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOARES, Marco César Prado. Design and development of microfermentors monitored by optical fiber sensor. 2020. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Mecânica Campinas, SP.