Advanced search
Start date
Betweenand

Development of fiber optic sensor for reflectance and light scattering monitoring for microfluidics applied to biotechnology

Grant number: 17/20445-8
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2018
Effective date (End): February 29, 2020
Field of knowledge:Engineering - Electrical Engineering - Electrical, Magnetic and Electronic Measurements, Instrumentation
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Eric Fujiwara
Grantee:Marco César Prado Soares
Home Institution: Faculdade de Engenharia Mecânica (FEM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Microfluidics is the science which studies the manipulation of small fluid samples in micrometric channels, and has the purpose of remodeling conventional procedures, using systems with more compact and versatile shapes. It is a knowledge field that is still in the beginning of its development, but that is already capable of offering a series of advantages in relation to traditional macroscopic processes. Microfluidics applied to biotechnology has been receiving increasing attention and, for the experiments achieve the best results, it is necessary to provide an efficient analysis method. Today, few monitoring and sensing techniques are applicable, and the use of microscopy with fluorescence markers is the main technique to evaluate cell growth in biological systems. Due to some advantages intrinsic to fiber optics, such as robustness, low cost and ease of operation, some studies on the use of fiber optic sensors applied to microfluidic systems have already begun. Based on preliminary results, which indicated the phenomenon of dynamic light scattering in Saccharomyces cerevisiae cultures operating in perfusion mode, this project seeks to apply fiber optic sensing to a microfluidic device, providing a simpler alternative to the professionals who deal with these equipments daily. For this, data of microbial kinetics will be obtained through the analysis of optical microscopy, and these data, in turn, will be compared to the parameters collected with the fiber optic sensor. In order to perform the experiments, the flow rates and the temperature of the device will be kept constant and, starting from a given initial concentration of cells, the response will be verified when the microorganisms are submitted to different concentrations of limiting substrate. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PRADO SOARES, MARCO CESAR; RODRIGUES, MATHEUS SANTOS; SCHENKEL, EGONT ALEXANDRE; PERLI, GABRIEL; ARITA SILVA, WILLIAN HIDEAK; GOMES, MATHEUS KAUE; FUJIWARA, ERIC; SUZUKI, CARLOS KENICHI. Evaluation of Silica Nanofluids in Static and Dynamic Conditions by an Optical Fiber Sensor. SENSORS, v. 20, n. 3 FEB 2020. Web of Science Citations: 0.
PRADO SOARES, MARCO CESAR; VIT, FRANCIELE FLORES; SUZUKI, CARLOS KENICHI; DE LA TORRE, LUCIMARA GAZIOLA; FUJIWARA, ERIC. Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters. SENSORS, v. 19, n. 11 JUN 1 2019. Web of Science Citations: 1.
MARCO CÉSAR PRADO SOARES; MATHEUS KAUÊ GOMES; EGONT ALEXANDRE SCHENKEL; MATHEUS DOS SANTOS RODRIGUES; CARLOS KENICHI SUZUKI; LUCIMARA GAZIOLA DE LA TORRE; ERIC FUJIWARA. EVALUATION OF SILICA NANOPARTICLE COLLOIDAL STABILITY WITH A FIBER OPTIC QUASI-ELASTIC LIGHT SCATTERING SENSOR. Brazilian Journal of Chemical Engineering, v. 36, n. 4, p. 1519-1534, Dez. 2019. Web of Science Citations: 0.
PRADO SOARES, MARCO CESAR; MENDES, BEATRIZ FERREIRA; SCHENKEL, EGONT ALEXANDRE; SANTOS, MURILO FERREIRA; FUJIWARA, ERIC; SUZUKI, CARLOS KENICHI. Kinetic and Thermodynamic Study in Pozzolanic Chemical Systems as an Alternative for Chapelle Test. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 21, n. 4 2018. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOARES, Marco César Prado. Design and development of microfermentors monitored by optical fiber sensor. 2020. Master's Dissertation - Universidade Estadual de Campinas, Faculdade de Engenharia Mecânica.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.