High throughput screening (HTS) biological platform for mitochondrial homeostasis evaluation in adherent cells

• Grant number: 17/21628-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2018
• End date: October 25, 2019
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Leonardo dos Reis Silveira
• Grantee: Tanes Imamura de Lima
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 16/23008-5 - Identification and functional characterization of protein bound to NCoR1 complex and associated with molecular control of mitochondrial biogenesis process, AP.TEM
• Associated scholarship(s): 19/11171-7 - Screening platform for the identification of molecular targets involved in mitochondrial homeostasis, BE.EP.PD

Abstract

The ability to synchronize mitochondrial signaling pathways to respond to energy fluctuations within the cell is a central aspect of mammalian homeostasis. In this context, mitochondria play a major role. Therefore, the molecular control of mitochondrial function represents a fundamental aspect of mammalian physiology and defects in the integrity of this system can lead to severe perturbations in cell homeostasis causing a vast spectrum of pathologies such as obesity and type 2 diabetes. The main goal of this project is to develop a high throughput screening (HTS) biological platform for mitochondrial homeostasis evaluation. Through this approach, we expect to identify novel biomolecules involved in the regulation of mitochondrial biogenesis by screening previously selected candidates from our lab (Thematic project-16/23008-5). Given the method versatility, we will also explore the HTS platform to screen compounds with mitochondrial function. Finally, we hope that this method can be used to generate knowledge with great impact in the field of metabolism and mitochondrial biology and ultimately contribute to the development of novel therapies for metabolic diseases such as obesity and type 2 diabetes.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, TANES I.; GUIMARAES, DIMITRIUS SANTIAGO P. S. F.; OLIVEIRA, ANDRE G.; ARAUJO, HYGOR; SPONTON, CARLOS H. G.; SOUZA-PINTO, NADJA C.; SAITO, ANGELA; FIGUEIRA, ANA CAROLINA M.; PALAMETA, SOLEDAD; BAJGELMAN, MARCIO CHAIM; et al. Opposing action of NCoR1 and PGC-1 alpha in mitochondrial redox homeostasis. Free Radical Biology and Medicine, v. 143, p. 203-208, NOV 1 2019. (16/23008-5, 17/21628-9, 17/04377-2)