Methylation pattern of Genomic DNA in mesial temporal lobe epilepsy associated with hippocampal sclerosis

• Grant number: 17/23954-0
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): April 01, 2018
• Effective date (End): February 29, 2020
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Iscia Teresinha Lopes Cendes
• Grantee: Jaqueline Cruz Geraldis
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/07559-3 - BRAINN - The Brazilian Institute of Neuroscience and Neurotechnology, AP.CEPID

Abstract

Epilepsy is a neurological disease with a high prevalence in the population. It is characterized by paroxysmal episodes of abnormal neuronal activity, resulting in epileptic seizures. Mesial temporal lobe epilepsy (MTLE), which may be associated with hippocampal sclerosis, is one of the most frequent and most severe types of epilepsy, since many patients present medically refractory seizures. In these patients surgical treatment may be a therapeutic alternative, which includes the surgical resection of abnormal cerebral tissue presenting epileptic activity. Such tissue allows for experiments to investigate gene expression regulation. Thus, the main objective of our work is to determine the overall methylation pattern of genomic DNA, the metilome, in tissue from patients with MTLE and compared it to normal brain tissue obtained from autopsy. In addition, we will correlate the metiolome data with the transcriptome and proteome profiles obtained from these patients in order to determine a more complete picture of mechanisms determining gene expression regulation in MTLE. We believe that our results will identify key molecular pathways that can be modulated or modified in future experiments, contributing to current efforts aimed at improving therapeutic interventions for patients with severe forms of epilepsy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

D.C.F. BRUNO; A. DONATTI; M. MARTIN; V.S. ALMEIDA; J.C. GERALDIS; F.S. OLIVEIRA; D.B. DOGINI; I. LOPES-CENDES. Circulating nucleic acids in the plasma and serum as potential biomarkers in neurological disorders. Brazilian Journal of Medical and Biological Research, v. 53, n. 10, 2020-08-17. (15/19768-1, 13/07559-3, 16/26172-0, 17/23954-0, 15/25607-0, 17/26167-0)

DO CANTO, AMANDA M.; DONATTI, AMANDA; GERALDIS, JAQUELINE C.; GODOI, ALEXANDRE B.; DA ROSA, DOUGLAS C.; LOPES-CENDES, ISCIA. Neuroproteomics in Epilepsy: What Do We Know so Far?. FRONTIERS IN MOLECULAR NEUROSCIENCE, v. 13, JAN 7 2021. (15/12960-4, 19/00048-0, 13/07559-3, 17/23954-0, 15/25607-0, 19/00213-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GERALDIS, Jaqueline Cruz. Methylation pattern of genomic DNA in mesial temporal lobe epilepsy associated with hippocampal sclerosis. 2020. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.