Comparative proteomic analysis of promastigote membrane proteins from l. (l.) amazonensis PH8 and LV79 strains

• Grant number: 17/26197-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2018
• End date: January 31, 2020
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Beatriz Simonsen Stolf
• Grantee: Fabia Tomie Tano
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Leishmaniasis, a group of diseases caused by the parasite of the genus Leishmania, is endemic in 98 countries and has an estimated annual incidence of more than 1 million cases. The infection can manifest itself in several clinical forms, which are divided into cutaneous, mucocutaneous and visceral. In Brazil, three dermotropic species are predominant, L. (V.) braziliensis, L. (V.) guyanensis and L. (L.) amazonensis. During its life cycle, the parasite presents itself as an extracellular promastigote transmitted by the insect vector and as an intracellular amastigote in the vertebrate host. The parasites express several virulence factors that influence their survival and infectivity. The amastigotes of the PH8 and LV79 strains of L. (L.) amazonensis have differences in virulence in mice and in protein expression. In vitro, PH8 promastigotes have greater macrophage adhesion and internalization even after a short period of interation. The analysis and comparison of Leishmania proteomes has been essential for determining the proteins involved in parasite virulence. Therefore, this project aims to compare the promastigote membrane proteome of PH8 and LV79 strains and to identify proteins possibly responsible for the differences in phagocytosis and infectivity between them. Subsequently, the importance of these virulence factors will be evaluated by their overexpression in LV79 strain, followed by in vitro infection assay in macrophages. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAPELLI-PEIXOTO, JANAINA; MULE, SIMON NGAO; TANO, FABIA TOMIE; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteomics and Leishmaniasis: Potential Clinical Applications. PROTEOMICS CLINICAL APPLICATIONS, v. 13, n. 6, AUG 2019. (17/26197-6, 17/04287-3, 14/26777-4, 14/06863-3, 18/18257-1, 18/15549-1, 17/04032-5)

TANO, FABIA TOMIE; TELLERIA, ERICH LOZA; REGO, FELIPE DUTRA; COELHO, FELIPE SOARES; DE REZENDE, ELOIZA; SOARES, RODRIGO PEDRO; TRAUB-CSEKO, YARA MARIA; STOLF, BEATRIZ SIMONSEN. Phenotypical Differences between Leishmania (Leishmania) amazonensis PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies. PATHOGENS, v. 12, n. 2, p. 13-pg., 2023-02-01. (18/14972-8, 21/08915-4, 17/26197-6)

TANO, FABIA TOMIE; BARBOSA, GUSTAVO ROLIM; DE REZENDE, ELOIZA; OLIVEIRA SOUZA, RODOLPHO ORNITZ; MUXEL, SANDRA MARCIA; SILBER, ARIEL MARIANO; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteome and morphological analysis show unexpected differences between promastigotes of Leishmania amazonensis PH8 and LV79 strains. PLoS One, v. 17, n. 8, p. 26-pg., 2022-08-23. (18/15549-1, 18/18257-1, 18/14972-8, 17/26197-6, 14/06863-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TANO, Fabia Tomie. Comparative analysis of the membrane proteome of Leishmania (Leishmania) amazonensis promastigotes of PH8 and LV79 strains.. 2021. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.